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Mouse polyclonal to NFATC2IP
In theory, the antibody could interact with isoform1 too since isoform2 is only a truncated version of isoform 1. Only isoform 2 has been tested (NFATC2IP transfected 293T cell lysate).
HumanPredicted to work with:
Mouse, Rat, Cynomolgus monkey
Recombinant fragment corresponding to Human NFATC2IP aa 1-138.
MSEPLQSVVDHMATHLGVSPSRILLLFGETELSPTATPRTLKLGVADIID CVVLTSSPEATETSQQLQLRVQGKEKHQTLEVSLSRDSPLKTLMSHYEEA MGLSGRKLSFFFDGTKLSGRELPADLGMESGDLIEVWG
- ICC/IF: HeLa cells.
WB: NFATC2IP transfected 293T cell lysate
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Constituent: 100% PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa.
Only isoform 2 has been tested (NFATC2IP transfected 293T cell lysate).
||Use a concentration of 10 µg/ml. Antibody purification with Protein A will be needed prior to use.
Plays a role in the inducible expression of cytokine genes in T-cells, especially by increasing NFAT-driven IL-4 production.
Contains 1 ubiquitin-like domain.
Methylation at the N-terminus by PRMT1 modulates interaction with the NFAT complex and results in augmented cytokine production.
Nucleus. Cytoplasm. TRAF1 is associated with a fraction of NFATC2IP in the cytoplasm and prevents its translocation to the nucleus.
Information by UniProt
- 45 kDa NF-AT-interacting protein antibody
- 45 kDa NFAT-interacting protein antibody
- cytoplasmic 2-interacting protein antibody
Immunocytochemistry/ Immunofluorescence - Anti-NFATC2IP antibody (ab169140)
Immunofluorescence analysis of permeabilized, paraformaldehyde-fixed HeLa cells, labeling NFATC2IP with ab169140 at 10 µg/ml.
Western blot - Anti-NFATC2IP antibody (ab169140)
All lanes : Anti-NFATC2IP antibody (ab169140) at 1 µg/ml
Lane 1 : NFATC2IP transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 15 kDa
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"