Question (15058) | Anti-NFATC4 antibody (ab3447)

Go to datasheet (ab3447)

Question

What methods for antigen retrieval would you recommend after formalin fixation for immunofluorescence?

Answer

Thank you for your enquiry. Unfortunately I do not have details of specific antigen retrieval that should be performed for this antibody. However, may I recommend you start with the mildest form of antigen retrieval in the form of heat mediated antigen retrieval. A time course should be performed to determine the best conditions to present the antigen to the antibody. We have excellent protocols located at the following web link: https://www.abcam.com/index.html?pageconfig=antigenretrieval I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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