Overview

  • Product name

    Anti-NFE2 antibody [EPR2824(2)]
    See all NFE2 primary antibodies
  • Description

    Rabbit monoclonal [EPR2824(2)] to NFE2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NFE2 aa 250-350. The exact sequence is proprietary.

  • Positive control

  • General notes

     

     

     This product was previously labelled as Nuclear Factor Erythroid Derived 2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab140598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
    • Tissue specificity

      Expressed in hematopoietic cells and also in colon and testis.
    • Sequence similarities

      Belongs to the bZIP family. CNC subfamily.
      Contains 1 bZIP domain.
    • Domain

      The PXY motifs are required for binding WW domains. PXY1 is required to promote transactivation of beta-globin and for hyperacetylation of histone H3, but not for binding to the HS2 promoter site.
    • Post-translational
      modifications

      Phosphorylated on serine residues (By similarity). In undifferentiated erythrocytes, phosphorylated by MAPK8 which then leads to ubiquitination and protein degradation.
      Sumoylated. Sumoylation is required for translocation to nuclear bodies PODs, anchoring to the gene loci, and transactivation of the beta-globin gene.
      Ubiquitinated mainly by 'Lys63'-linked ubiquitin. Polyubiquitination with 'Lys63'-linked ubiquitin by ITCH retains NFE2 in the cytoplasm preventing its transactivation activity. In undifferentiated erythrocyte, ubiquitinated after MAPK8-mediatd phosphorylation leading to protein degradation.
    • Cellular localization

      Nucleus > PML body. Cytoplasm. The sumoylated form locates to the nuclear bodies PML oncogenic domains (PODs). Translocated to the cytoplasm through interaction with ITCH.
    • Information by UniProt
    • Database links

    • Alternative names

      • erythroid-derived 2 45 kDa subunit antibody
      • Leucine zipper protein NF-E2 antibody
      • Leucine zipper protein NFE2 antibody
      • NF E2 antibody
      • nfe2 antibody
      • NFE2_HUMAN antibody
      • Nuclear factor (erythroid derived 2), 45kDa antibody
      • nuclear factor (erythroid-derived 2), 45kD antibody
      • Nuclear factor antibody
      • nuclear factor, erythroid 2 antibody
      • Nuclear factor, erythroid derived 2 45 kDa subunit antibody
      • p45 antibody
      • p45 NF-E2 antibody
      • p45 NFE2 antibody
      • Transcription factor NF-E2 45 kDa subunit antibody
      • Transcription factor NFE2 45 kDa subunit antibody
      see all

    Images

    • All lanes : Anti-NFE2 antibody [EPR2824(2)] (ab140598) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : U937 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 41 kDa

    • Immunohistochemical analysis of paraffin embedded Human colon tissue labelling NFE2 with ab140598 antibody at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human testis tissue labelling NFE2 with ab140598 antibody at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling NFE2 with ab140598 antibody at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    See 1 Publication for this product

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