ab41851 gave a positive result in the following Human Whole Cell Lysates: Hela, Jurkat, A431, HEK293, HepG2 and MCF-7.
In addition, ab41851 gave a postive result in the following Tissue Lysates: Human Liver, Mouse Liver, Rat Liver (Data not shown), Rat Brain (Data not shown).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-Fr: See Abreview for dilution recommendations
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 56 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Belongs to the CTF/NF-I family. Contains 1 CTF/NF-I DNA-binding domain.
ICC/IF image of ab41851 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab41851, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Frozen sections) - Anti-NFIA antibody (ab41851)This image is courtesy of an abreview submitted by Dr Sophie Pezet
Ab41851 staining NFIA in motorneurons of rat brain tissue sections by IHC-Fr. Sections were PFA fixed and incubated with ab41851, diluted 1/100, for 24 hours at 4°C. Chromeo™ 488 conjugated goat polyclonal to rabbit IgG ab60314, diluted 1/2000, was used as the secondary antibody.