Product nameAnti-NFIB / NF1B2 antibody
See all NFIB / NF1B2 primary antibodies
DescriptionRabbit polyclonal to NFIB / NF1B2
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Chicken, Cow
Synthetic peptide within Human NFIB/ NF1B2 aa 325-375. The exact sequence is proprietary. NP_005587.2
Database link: O00712
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 47 kDa.|
FunctionRecognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Sequence similaritiesBelongs to the CTF/NF-I family.
Contains 1 CTF/NF-I DNA-binding domain.
- Information by UniProt
- CCAAT Box Binding Transcription Factor antibody
- CCAAT-box-binding transcription factor antibody
- CTF antibody
All lanes : Anti-NFIB / NF1B2 antibody (ab264332) at 0.1 µg/ml
Lane 1 : HeLa human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 30 seconds
NFIB / NF1B2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264332 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264332 at 1 μg/ml.
Lane 1: ab264332 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.
Exposure time: 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264332 has not yet been referenced specifically in any publications.