Recombinant
RabMAb

Recombinant Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

Overview

  • Product name

    Anti-NFIB / NF1B2 antibody [EPR14122]
    See all NFIB / NF1B2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14122] to NFIB / NF1B2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NFIB/ NF1B2 aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: O00712

  • Positive control

    • HeLa, rat C6, rat PC12 and mouse NIH 3T3 cell lysates; Human fetal brain lysate; Human breast and Mouse liver tissue; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14122
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 47,53,55 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.
IP 1/30 - 1/50.

Target

  • Function

    Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
  • Sequence similarities

    Belongs to the CTF/NF-I family.
    Contains 1 CTF/NF-I DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCAAT Box Binding Transcription Factor antibody
    • CCAAT-box-binding transcription factor antibody
    • CTF antibody
    • HMGIC/NFIB antibody
    • NF-I/B antibody
    • NF1-B antibody
    • NF1B antibody
    • NF1B2 antibody
    • NFI-B antibody
    • NFI-RED antibody
    • Nfib antibody
    • NFIB_HUMAN antibody
    • NFIB2 antibody
    • NFIB3 antibody
    • Nuclear factor 1 B-type antibody
    • Nuclear factor 1/B antibody
    • Nuclear Factor 1B antibody
    • Nuclear Factor I B antibody
    • Nuclear factor I/B antibody
    • TGGCA Binding Protein antibody
    • TGGCA-binding protein antibody
    • TRANSCRIPTION FACTOR NFIB antibody
    see all

Images

  • Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/5000 dilution + Human fetal brain at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • ab186738 staining NFIB / NF1B2 in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/70. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/5000 dilution + HeLa cell lysate at 10 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 47 kDa
    Observed band size: 47, 53, 55 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/1000 dilution

    Lane 1 : rat C6 cell lysate
    Lane 2 : rat PC12 cell lysate
    Lane 3 : mouse NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • Immunohistochemical analysis of paraffin-embedded, Human breast tissue labeling NFIB / NF1B2 with ab186738 at a 1/500 dilution. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded, mouse liver tissue labeling NFIB / NF1B2 with ab186738 at a 1/500 dilution. Counter stained with hematoxylin.

  • Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling NFIB / NF1B2 with ab186738 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining.

  • Western blot analysis on immunoprecipitation from 1) Human fetal brain lysate and 2) PBS, labeling NFIB / NF1B2 using ab186738 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

References

ab186738 has not yet been referenced specifically in any publications.

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