Recombinant Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14122] to NFIB / NF1B2 - BSA and Azide free
- Suitable for: IP, IHC-P, ICC/IF, Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free
See all NFIB / NF1B2 primary antibodies -
Description
Rabbit monoclonal [EPR14122] to NFIB / NF1B2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, ICC/IF, Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250892 is the carrier-free version of ab186738.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14122 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
- Alexa Fluor® 488 Anti-NFIB / NF1B2 antibody [EPR14122] (ab216931)
- PE Anti-NFIB / NF1B2 antibody [EPR14122] (ab305979)
- APC Anti-NFIB / NF1B2 antibody [EPR14122] (ab305980)
- HRP Anti-NFIB / NF1B2 antibody [EPR14122] (ab305981)
- Alexa Fluor® 647 Anti-NFIB / NF1B2 antibody [EPR14122] (ab310047)
- Alexa Fluor® 594 Anti-NFIB / NF1B2 antibody [EPR14122] (ab310434)
- Alexa Fluor® 555 Anti-NFIB / NF1B2 antibody [EPR14122] (ab311961)
- Alexa Fluor® 568 Anti-NFIB / NF1B2 antibody [EPR14122] (ab312433)
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 47,53,55 kDa (predicted molecular weight: 47 kDa).
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Notes |
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IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 47,53,55 kDa (predicted molecular weight: 47 kDa). |
Target
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Function
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. -
Sequence similarities
Belongs to the CTF/NF-I family.
Contains 1 CTF/NF-I DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4781 Human
- Entrez Gene: 18028 Mouse
- Entrez Gene: 29227 Rat
- Omim: 600728 Human
- SwissProt: O00712 Human
- SwissProt: P97863 Mouse
- Unigene: 644095 Human
- Unigene: 317947 Mouse
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Alternative names
- CCAAT Box Binding Transcription Factor antibody
- CCAAT-box-binding transcription factor antibody
- CTF antibody
see all
Images
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Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/5000 dilution + Human fetal brain at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDaThis data was developed using ab186738, the same antibody clone in a different buffer formulation.
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This data was developed using ab186738, the same antibody clone in a different buffer formulation.
ab186738 staining NFIB / NF1B2 in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/70. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/5000 dilution + HeLa cell lysate at 10 µg
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47, 53, 55 kDa why is the actual band size different from the predicted?This data was developed using ab186738, the same antibody clone in a different buffer formulation.
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All lanes : Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDaThis data was developed using ab186738, the same antibody clone in a different buffer formulation.
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This data was developed using ab186738, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, Human breast tissue labeling NFIB / NF1B2 with ab186738 at a 1/500 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab186738, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, mouse liver tissue labeling NFIB / NF1B2 with ab186738 at a 1/500 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab186738, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling NFIB / NF1B2 with ab186738 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining.
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This data was developed using ab186738, the same antibody clone in a different buffer formulation.
Western blot analysis on immunoprecipitation from 1) Human fetal brain lysate and 2) PBS, labeling NFIB / NF1B2 using ab186738 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab250892 has been referenced in 1 publication.
- Vancamp P et al. Gender-specific effects of transthyretin on neural stem cell fate in the subventricular zone of the adult mouse. Sci Rep 9:19689 (2019). PubMed: 31873158