• Product name
    Anti-NFIB / NF1B2 antibody
    See all NFIB / NF1B2 primary antibodies
  • Description
    Rabbit polyclonal to NFIB / NF1B2
  • Host species
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Hamster
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 402-415 of Human NF1B2

  • Positive control
    • MG-63 nuclear extract.



Our Abpromise guarantee covers the use of ab11989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). A doublet may be seen in some cell lines. For optimal results, primary antibody incubations should be performed at room temperature (see recommended protocol). The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background.


  • Function
    Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
  • Sequence similarities
    Belongs to the CTF/NF-I family.
    Contains 1 CTF/NF-I DNA-binding domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • CCAAT Box Binding Transcription Factor antibody
    • CCAAT-box-binding transcription factor antibody
    • CTF antibody
    • HMGIC/NFIB antibody
    • NF-I/B antibody
    • NF1-B antibody
    • NF1B antibody
    • NF1B2 antibody
    • NFI-B antibody
    • NFI-RED antibody
    • Nfib antibody
    • NFIB_HUMAN antibody
    • NFIB2 antibody
    • NFIB3 antibody
    • Nuclear factor 1 B-type antibody
    • Nuclear factor 1/B antibody
    • Nuclear Factor 1B antibody
    • Nuclear Factor I B antibody
    • Nuclear factor I/B antibody
    • TGGCA Binding Protein antibody
    • TGGCA-binding protein antibody
    see all


  • NF1B2 detection by Western blot analysis. NF1B2 is detected in Rat Liver cells using NF1B2 rabbit polyclonal antibody at a 1/1000 dilution (lane 1). The signal is eliminated by adding the immunizing peptide to the reaction mixture (lane 2). NF1B2 detection by Western blot analysis. NF1B2 is detected in Rat Liver cells using NF1B2 rabbit polyclonal antibody at a 1/1000 dilution (lane 1). The signal is eliminated by adding the immunizing peptide to the reaction mixture (lane 2).
  • ab11989 staining of NFIB/NF1B2 in mouse cortex E15. PFA-fixed paraffin-embedded sections of mouse cortex E15 were incubated with ab11989 (1/1000) for 18 hours at 4°C. Antigen retrieval was performed by heat induction in sodium citrate buffer. Please see accompanying abreview for additional information.

    See Abreview


This product has been referenced in:
  • Stringer BW  et al. Nuclear factor one B (NFIB) encodes a subtype-specific tumour suppressor in glioblastoma. Oncotarget 7:29306-20 (2016). WB . Read more (PubMed: 27083054) »
  • Dellago H  et al. High levels of oncomiR-21 contribute to the senescence-induced growth arrest in normal human cells and its knock-down increases the replicative lifespan. Aging Cell 12:446-58 (2013). WB . Read more (PubMed: 23496142) »

See all 4 Publications for this product

Customer reviews and Q&As

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The credit note ID for order PO-13779 is XXXXX.

The FOCR for order number PO-14643 is 1111586.

I hope this information will be helpful.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Mouse cortex E15)
Mouse cortex E15
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Yes - 0.025% TX100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jul 23 2008

Thank you for your e-mail. The source of the antibody told us " I am not sure if the 3 isoforms are expressed simultaneously in rat liver nuclear extract and I think that's not the case". We hope this information will clarify the reason for 1 band...

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The source of the antibody ab11989 has informed us " The antibody is raised against the peptide YVPSYDPSSPQTSQ. From our blast results, it seems the antibody should recognize all 3 isoforms". We hope this information will help you, please do not hes...

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