Product nameAnti-NFIC / CTF antibody [440C4a]
See all NFIC / CTF primary antibodies
DescriptionMouse monoclonal [440C4a] to NFIC / CTF
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human NFIC/ CTF.
Protein previously labeled as NFIC.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS
Concentration information loading...
PurityProtein G purified
Purification notesab54403 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Our Abpromise guarantee covers the use of ab54403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 36 kDa for the recombinant fragment (predicted molecular weight of the full length protein: 56 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRecognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Sequence similaritiesBelongs to the CTF/NF-I family.
Contains 1 CTF/NF-I DNA-binding domain.
- Information by UniProt
- 1110019L22Rik antibody
- 1500041O16Rik antibody
- AA589446 antibody
Anti-NFIC / CTF antibody [440C4a] (ab54403) + immunised recombinant protein
Predicted band size: 56 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
ab54403 has not yet been referenced specifically in any publications.