Anti-NFIC/CTF antibody (ab245597)
Key features and details
- Rabbit polyclonal to NFIC/CTF
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NFIC/CTF antibody
See all NFIC/CTF primary antibodies -
Description
Rabbit polyclonal to NFIC/CTF -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Pig -
Immunogen
Synthetic peptide within Human NFIC/CTF aa 200-250. The exact sequence is proprietary. EAW69324.1
Database link: P08651 -
Positive control
- IHC-P: Human breast carcinoma and mouse renal cell carcinoma tissue. WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab245597 was affinity purified using an epitope specific to NFIC/CTF immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 56 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Predicted molecular weight: 56 kDa. |
Target
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Function
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. -
Sequence similarities
Belongs to the CTF/NF-I family.
Contains 1 CTF/NF-I DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4782 Human
- Entrez Gene: 18029 Mouse
- Omim: 600729 Human
- SwissProt: P08651 Human
- SwissProt: P70255 Mouse
- SwissProt: P21999 Pig
- Unigene: 170131 Human
- Unigene: 426936 Mouse
see all -
Alternative names
- 1110019L22Rik antibody
- 1500041O16Rik antibody
- AA589446 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for NFIC/CTF with ab245597 at a 1/200 dilution in immunohistochemical analysis. DAB staining. Counterstained using hematoxylin (blue).
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Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for NFIC/CTF with ab245597 at a 1/200 dilution in immunohistochemical analysis. DAB staining.
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All lanes : Anti-NFIC/CTF antibody (ab245597) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 3 minutes -
NFIC/CTF was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245597 used for IP at 6 µg/mg lysate. For WB 1 µg/ml.
Lane 1: ab245597 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.Chemiluminescence detection: 3 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab245597 has been referenced in 2 publications.
- Hwang JH et al. CREB5 reprograms FOXA1 nuclear interactions to promote resistance to androgen receptor-targeting therapies. Elife 11:N/A (2022). PubMed: 35550030
- Liang X et al. ECRG4 Represses Cell Proliferation and Invasiveness via NFIC/OGN/NF-?B Signaling Pathway in Bladder Cancer. Front Genet 11:846 (2020). PubMed: 32922434