Overview

  • Product name

  • Description

    Rabbit polyclonal to NFIC/CTF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide within Human NFIC/CTF aa 200-250. The exact sequence is proprietary. EAW69324.1
    Database link: P08651

  • Positive control

    • IHC-P: Human breast carcinoma and mouse renal cell carcinoma tissue. WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245597 was affinity purified using an epitope specific to NFIC/CTF immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 56 kDa.

Target

  • Function

    Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
  • Sequence similarities

    Belongs to the CTF/NF-I family.
    Contains 1 CTF/NF-I DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1110019L22Rik antibody
    • 1500041O16Rik antibody
    • AA589446 antibody
    • AI746521 antibody
    • CAAT box transcription factor antibody
    • CCAAT binding transcription factor antibody
    • CCAAT box binding transcription factor antibody
    • CCAAT-box-binding transcription factor antibody
    • CNFI C antibody
    • CTF antibody
    • CTF5 antibody
    • MGC137374 antibody
    • MGC20153 antibody
    • NF I antibody
    • NF I/C antibody
    • NF-I/C antibody
    • NF1 C antibody
    • NF1-C antibody
    • NF1C antibody
    • NFI antibody
    • NFI-C antibody
    • NFI/C antibody
    • NFIC antibody
    • NFIC_HUMAN antibody
    • Nuclear factor 1 antibody
    • Nuclear factor 1 C type antibody
    • Nuclear factor 1 C-type antibody
    • Nuclear factor 1/C antibody
    • Nuclear factor I/C (CCAAT binding transcription factor) antibody
    • Nuclear factor I/C antibody
    • TGGCA binding protein antibody
    • TGGCA-binding protein antibody
    • Transcription factor NFIC antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for NFIC/CTF with ab245597 at a 1/200 dilution in immunohistochemical analysis. DAB staining. Counterstained using hematoxylin (blue).

  • Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for NFIC/CTF with ab245597 at a 1/200 dilution in immunohistochemical analysis. DAB staining.

  • All lanes : Anti-NFIC/CTF antibody (ab245597) at 0.04 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 56 kDa


    Exposure time: 3 minutes
  • NFIC/CTF was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

    ab245597 used for IP at 6 µg/mg lysate. For WB 1 µg/ml.

    Lane 1: ab245597 IP in HeLa whole cell lysate.
    Lane 2: Control IgG in HeLa whole cell lysate.

    Chemiluminescence detection: 3 seconds.

References

ab245597 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab245597.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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