Key features and details
- Rabbit polyclonal to NFIL3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NFIL3 antibody
See all NFIL3 primary antibodies
DescriptionRabbit polyclonal to NFIL3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human NFIL3 aa 133-462.
AYAQEIQKLSNSTAVYFQDYQTSKSNVSSFVDEHEPSMVSSSCISVIKHS PQSSLSDVSEVSSVEHTQESSVQGSCRSPENKFQIIKQEPMELESYTREP RDDRGSYTASIYQNYMGNSFSGYSHSPPLLQVNRSSSNSPRTSETDDGVV GKSSDGEDEQQVPKGPIHSPVELKHVHATVVKVPEVNSSALPHKLRIKAK AMQIKVEAFDNEFEATQKLSSPIDMTSKRHFELEKHSAPSMVHSSLTPFS VQVTNIQDWSLKSEHWHQKELSGKTQNSFKTGVVEMKDSGYKVSDPENLY LKQGIANLSAEVVSLKRLIATQPISASDSG
Database link: Q16649
- WB: HepG2, HEK-293T and HeLa whole cell lysates. IHC-P: Human thyroid and adrenal gland tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230090 in the following tested applications.
|WB||1/1000 - 1/5000. Predicted molecular weight: 51 kDa.|
|IHC-P||1/20 - 1/200.|
FunctionActs as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity). Represses transcriptional activity of PER1 (By similarity). Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity). Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity). Protects pro-B cells from programmed cell death.
Tissue specificityExpressed in bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle.
Sequence similaritiesBelongs to the bZIP family. NFIL3 subfamily.
Contains 1 bZIP domain.
- Information by UniProt
- E4 promoter binding protein 4 antibody
- E4 promoter-binding protein 4 antibody
- E4BP 4 antibody
All lanes : Anti-NFIL3 antibody (ab230090) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
Paraffin-embedded human thyroid tissue stained for NFIL3 using ab230090 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human adrenal gland tissue stained for NFIL3 using ab230090 at 1/100 dilution in immunohistochemical analysis.
ab230090 has not yet been referenced specifically in any publications.