Key features and details
- Rabbit polyclonal to NFIL3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NFIL3 antibody
See all NFIL3 primary antibodies
DescriptionRabbit polyclonal to NFIL3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region within amino acids 412-462 of Human NFIL3 (NP_005375.2).
- Whole cell lysate from HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab93785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 51 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionActs as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity). Represses transcriptional activity of PER1 (By similarity). Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity). Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity). Protects pro-B cells from programmed cell death.
Tissue specificityExpressed in bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle.
Sequence similaritiesBelongs to the bZIP family. NFIL3 subfamily.
Contains 1 bZIP domain.
- Information by UniProt
- E4 promoter binding protein 4 antibody
- E4 promoter-binding protein 4 antibody
- E4BP 4 antibody
All lanes : Anti-NFIL3 antibody (ab93785) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 3 minutes
Detection of NFIL3 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab93785 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab93785 has been referenced in 2 publications.
- Karthik IP et al. E4BP4/NFIL3 modulates the epigenetically repressed RAS effector RASSF8 function through histone methyltransferases. J Biol Chem 293:5624-5635 (2018). PubMed: 29467226
- Ning H et al. Conversion of adipose-derived stem cells into natural killer-like cells with anti-tumor activities in nude mice. PLoS One 9:e106246 (2014). WB ; Human . PubMed: 25162225