Key features and details
- Rabbit polyclonal to NFIX/CTF
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-NFIX/CTF antibody
See all NFIX/CTF primary antibodies
DescriptionRabbit polyclonal to NFIX/CTF
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human
Recombinant fragment corresponding to Rat NFIX/CTF aa 13-298. (N-terminal His-tag and GST-tag; Expressed in E.coli).
HPFIEALLPHVRAFSYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLG EKPEIKQKWASRLLAKLRKDIRPEFREDFVLTITGKKPPCCVLSNPDQKG KIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLYKSPQCSNPGLC VQPHHIGVTIKELDLYLAYFVHTPESGQSDSSNQQGDADIKPLPNGHLSF QDCFVTSGVWNVTELVRVSQTPVATASGPNFSLADLESPSYYNINQVTLG RRSITSPPSTSTTKRPKSIDDSEMESPVDDVFYPGT
Database link: F2Z3R4
- WB: Rat serum; Recombinant rat NFIX/CTF protein IHC-P: Rat skin tissue.
Product previously known as Rabbit polyclonal to NFIX.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231324 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 49 kDa.|
FunctionRecognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Sequence similaritiesBelongs to the CTF/NF-I family.
Contains 1 CTF/NF-I DNA-binding domain.
- Information by UniProt
- CCAAT box binding transcription factor antibody
- CCAAT-box-binding transcription factor antibody
- CTF antibody
Formalin-fixed, paraffin-embedded rat skin tissue stained for NFIX/CTF with ab231324 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-NFIX/CTF antibody (ab231324) at 1 µg/ml + Rat serum.
Predicted band size: 49 kDa
Anti-NFIX/CTF antibody (ab231324) at 1 µg/ml + Recombinant rat NFIX protein
Predicted band size: 49 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231324 has not yet been referenced specifically in any publications.