Product nameAnti-NFkB c Rel antibody
See all NFkB c Rel primary antibodies
DescriptionRabbit polyclonal to NFkB c Rel
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human NFkB c Rel aa 569-619. The exact sequence is proprietary. NP_002899.1
Database link: Q04864
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264238 in the following tested applications.
|WB||1/2000 - 1/10000. Predicted molecular weight: 69 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
Relevancec Rel is a protooncogene encoding a subunit of the NF kappa B family of transcription factors. In B and T cells, it is involved in the regulation of genes controlling cell division and immune function.
- C Rel antibody
- C Rel protein antibody
- C rel proto oncogene protein antibody
All lanes : Anti-NFkB c Rel antibody (ab264238) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 30 seconds
NFkB c Rel was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264238 at 3 μg per reaction. Western blot was performed on the immunoprecipitates using ab264238 at 1 μg/ml.
Lane 1: ab264238 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.
Exposure time: 30 seconds.
ab264238 has not yet been referenced specifically in any publications.