Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [3H31L48] to NFkB Inducing Kinase NIK
- Suitable for: WB, ICC/IF
- Reacts with: Human
Product nameAnti-NFkB Inducing Kinase NIK antibody [3H31L48]
See all NFkB Inducing Kinase NIK primary antibodies
DescriptionRabbit monoclonal [3H31L48] to NFkB Inducing Kinase NIK
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
- 293 cells transfected with NIK; FLAG tagged NIK transfected 293 lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 10 - 20 µg/ml. Predicted molecular weight: 104 kDa.|
|ICC/IF||Use a concentration of 4 - 6 µg/ml.|
FunctionLymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
Tissue specificityWeakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
modificationsAutophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
- Information by UniProt
- MAP3K14 antibody
- FTDCR1B antibody
- HS antibody
Lane 1 : anti FLAG tag antibody
Lane 2 : Anti-NFkB Inducing Kinase NIK antibody [3H31L48] (ab203568) at 15 µg/ml
All lanes : Flag tagged NIK transfected 293 cell lysate
Predicted band size: 104 kDa
Immunohistochemical analysis of non-transfected (left) and NIK transfected 293 cells (right) labeling NIK using ab203568 at 5 μg/mL. Alexa Fluor® 488 Goat anti-Rabbit at 1/1000 dilution was used as secondary antibody (green). Hoescht staining of nucleus (blue).
ab203568 has been referenced in 1 publication.
- Li XJ et al. Bergapten exerts inhibitory effects on diabetes-related osteoporosis via the regulation of the PI3K/AKT, JNK/MAPK and NF-?B signaling pathways in osteoprotegerin knockout mice. Int J Mol Med N/A:N/A (2016). WB ; Mouse . PubMed: 27840967