The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/1000. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
1/100 - 1/500.
Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.
Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization. Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat brain tissue labeling NFkB Inducing Kinase NIK with ab216409 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Western blot - Anti-NFkB Inducing Kinase NIK antibody (ab216409)
All lanes : Anti-NFkB Inducing Kinase NIK antibody (ab216409) at 1/200 dilution
Lane 1 : Mouse kidney lysate Lane 2 : Mouse brain lysate
Secondary All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HR at 1/3000 dilution