Key features and details
- Rabbit polyclonal to NFkB Inducing Kinase NIK
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-NFkB Inducing Kinase NIK antibody
See all NFkB Inducing Kinase NIK primary antibodies
DescriptionRabbit polyclonal to NFkB Inducing Kinase NIK
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
- Rat brain tissue; mouse brain and kidney lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).|
|IHC-P||1/100 - 1/500.|
FunctionLymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
Tissue specificityWeakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
modificationsAutophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
- Information by UniProt
- MAP3K14 antibody
- FTDCR1B antibody
- HS antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat brain tissue labeling NFkB Inducing Kinase NIK with ab216409 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
All lanes : Anti-NFkB Inducing Kinase NIK antibody (ab216409) at 1/200 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse brain lysate
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HR at 1/3000 dilution
Predicted band size: 104 kDa
ab216409 has not yet been referenced specifically in any publications.