• Product name

    Anti-NFkB Inducing Kinase NIK antibody
    See all NFkB Inducing Kinase NIK primary antibodies
  • Description

    Rabbit polyclonal to NFkB Inducing Kinase NIK
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human NFkB Inducing Kinase NIK aa 795-947.
    Database link: Q99558



Our Abpromise guarantee covers the use of ab7204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 23301098
WB 1/2000.
IP 1/500.


  • Function

    Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
  • Tissue specificity

    Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational

    Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
    Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • MAP3K14 antibody
    • FTDCR1B antibody
    • HS antibody
    • HsNIK antibody
    • M3K14_HUMAN antibody
    • Map3k14 antibody
    • Mitogen activated protein kinase kinase kinase 14 antibody
    • Mitogen-activated protein kinase kinase kinase 14 antibody
    • NF kappa beta inducing kinase antibody
    • NF-kappa-beta-inducing kinase antibody
    • NIK antibody
    • Serine/threonine-protein kinase NIK antibody
    see all


  • All lanes : Anti-NFkB Inducing Kinase NIK antibody (ab7204)

    Lane 1 : macrophages lysate
    Lane 2 : NIK transfected cell lysate

    Western blot detection of NIK in 1, macrophage and 2, NIK transfected cells by anti-human NIK.

  • ICC/IF image of ab7204 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Wang X  et al. Tunicamycin suppresses breast cancer cell growth and metastasis via regulation of the protein kinase B/nuclear factor-?B signaling pathway. Oncol Lett 15:4137-4142 (2018). Read more (PubMed: 29541178) »
  • Jung JU  et al. NIK/MAP3K14 Regulates Mitochondrial Dynamics and Trafficking to Promote Cell Invasion. Curr Biol N/A:N/A (2016). ICC/IF ; Human . Read more (PubMed: 27889261) »
See all 9 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10%)
Mouse Cell lysate - whole cell (colon)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Liwei Xie

Verified customer

Submitted Apr 23 2014

Western blot
Human Cell lysate - whole cell (HEK293)
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jul 16 2012


Thank you for your secondary. Ab7092 - Goat polyclonal to Rat IgG H&L Pre-Adsorbed - is a secondary antibody and is not a positive control for ab7204. Ab7204 was characterized for application in Western blotting using NIK transfected cell lysate which would be recommended as a positive control. There is an image showing this on the online datasheet. Please contact us again if you have any additional questions.

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Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with mouse has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. We currently do not have a mouse NIK antibody. To see is another company may have what you are looking for, I suggest that you click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249). If you have ant more questions, please contact us again.

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We haven't tested endogenous NIK but one of our customers has got this to reliably work for detecting endogenous NIK.

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