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Cell Biology Apoptosis Intracellular Kinases
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Anti-NFkB Inducing Kinase NIK antibody - N-terminal (ab137671)

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Western blot - Anti-NFkB Inducing Kinase NIK antibody - N-terminal (ab137671)

    Key features and details

    • Rabbit polyclonal to NFkB Inducing Kinase NIK - N-terminal
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Product image
    Recombinant Human NFkB Inducing Kinase NIK protein (ab61262)

    View more associated products

    Overview

    • Product name

      Anti-NFkB Inducing Kinase NIK antibody - N-terminal
      See all NFkB Inducing Kinase NIK primary antibodies
    • Description

      Rabbit polyclonal to NFkB Inducing Kinase NIK - N-terminal
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Recombinant fragment, corresponding to a region within N terminal amino acids 1-213 of Human NFkB Inducing Kinase NIK (Uniprot ID: Q99558).

    • Positive control

      • A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • Kinases
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Other Kinases
      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • NFkB Pathway

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Hep G2 cytoplasmic extract lysate (ab14659)
      • HeLa whole cell lysate (ab29545)
      • Raji whole cell lysate (ab30124)
      • A-431 whole cell lysate (ab7909)
      • MOLT-4 whole cell lysate (ab7912)
    • Recombinant Protein

      • Recombinant Human NFkB Inducing Kinase NIK protein (ab61262)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab137671 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Rat
    Application Abreviews Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 104 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 104 kDa.

    Target

    • Function

      Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
    • Tissue specificity

      Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
    • Sequence similarities

      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
      Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession Q99558 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9020 Human
      • Entrez Gene: 53859 Mouse
      • Entrez Gene: 360640 Rat
      • Omim: 604655 Human
      • SwissProt: Q99558 Human
      • SwissProt: Q9WUL6 Mouse
      • Unigene: 404183 Human
      • Unigene: 668927 Human
      • Unigene: 735695 Human
      • Unigene: 158981 Mouse
      • Unigene: 88085 Rat
      see all
    • Alternative names

      • MAP3K14 antibody
      • FTDCR1B antibody
      • HS antibody
      • HsNIK antibody
      • M3K14_HUMAN antibody
      • Map3k14 antibody
      • Mitogen activated protein kinase kinase kinase 14 antibody
      • Mitogen-activated protein kinase kinase kinase 14 antibody
      • NF kappa beta inducing kinase antibody
      • NF-kappa-beta-inducing kinase antibody
      • NIK antibody
      • Serine/threonine-protein kinase NIK antibody
      see all

    Images

    • Western blot - Anti-NFkB Inducing Kinase NIK antibody - N-terminal (ab137671)
      Western blot - Anti-NFkB Inducing Kinase NIK antibody - N-terminal (ab137671)
      All lanes : Anti-NFkB Inducing Kinase NIK antibody - N-terminal (ab137671) at 1/1000 dilution

      Lane 1 : H1299 whole cell lysate
      Lane 2 : HepG2 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 104 kDa



      7.5% SDS PAGE

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab137671? Please let us know so that we can cite the reference in this datasheet.

    ab137671 has not yet been referenced specifically in any publications.

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