Anti-NFkB Inducing Kinase NIK antibody - N-terminal (ab137671)
Key features and details
- Rabbit polyclonal to NFkB Inducing Kinase NIK - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-NFkB Inducing Kinase NIK antibody - N-terminal
See all NFkB Inducing Kinase NIK primary antibodies -
Description
Rabbit polyclonal to NFkB Inducing Kinase NIK - N-terminal -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Recombinant fragment, corresponding to a region within N terminal amino acids 1-213 of Human NFkB Inducing Kinase NIK (Uniprot ID: Q99558).
-
Positive control
- A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
WB |
Human
|
All applications |
Mouse
Rat
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 104 kDa.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 104 kDa. |
Target
-
Function
Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. -
Tissue specificity
Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 9020 Human
- Entrez Gene: 53859 Mouse
- Entrez Gene: 360640 Rat
- Omim: 604655 Human
- SwissProt: Q99558 Human
- SwissProt: Q9WUL6 Mouse
- Unigene: 404183 Human
- Unigene: 668927 Human
see all -
Alternative names
- MAP3K14 antibody
- FTDCR1B antibody
- HS antibody
see all
Images
-
All lanes : Anti-NFkB Inducing Kinase NIK antibody - N-terminal (ab137671) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 104 kDa
7.5% SDS PAGE
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab137671 has not yet been referenced specifically in any publications.