Anti-NFkB Inducing Kinase NIK antibody - N-terminal (ab137671)
Key features and details
- Rabbit polyclonal to NFkB Inducing Kinase NIK - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-NFkB Inducing Kinase NIK antibody - N-terminal
See all NFkB Inducing Kinase NIK primary antibodies -
Description
Rabbit polyclonal to NFkB Inducing Kinase NIK - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment, corresponding to a region within N terminal amino acids 1-213 of Human NFkB Inducing Kinase NIK (Uniprot ID: Q99558).
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Positive control
- A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 104 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 104 kDa. |
Target
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Function
Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. -
Tissue specificity
Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 9020 Human
- Entrez Gene: 53859 Mouse
- Entrez Gene: 360640 Rat
- Omim: 604655 Human
- SwissProt: Q99558 Human
- SwissProt: Q9WUL6 Mouse
- Unigene: 404183 Human
- Unigene: 668927 Human
see all -
Alternative names
- MAP3K14 antibody
- FTDCR1B antibody
- HS antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137671 has not yet been referenced specifically in any publications.