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Cell Biology Apoptosis Intracellular NFkB p52
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Anti-NFkB p100/NFKB2 antibody (ab131539)

  • Datasheet
Submit a review Submit a question References (3)

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Western blot - Anti-NFkB p100/NFKB2 antibody (ab131539)
  • Immunocytochemistry/ Immunofluorescence - Anti-NFkB p100/NFKB2 antibody (ab131539)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (ab131539)

Key features and details

  • Rabbit polyclonal to NFkB p100/NFKB2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-NFkB p100/NFKB2 antibody
    See all NFkB p100/NFKB2 primary antibodies
  • Description

    Rabbit polyclonal to NFkB p100/NFKB2
  • Host species

    Rabbit
  • Specificity

    The antibody detects endogenous level of total NFkB-p100 protein.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human NFkB p100/NFKB2 aa 870-874 conjugated to keyhole limpet haemocyanin.
    Sequence:

    S-Q-S-V-E


    Database link: Q00653
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa cells; HT29 cell extract; Human lung carcinoma tissue
  • General notes

    This antibody detects sites on p100 only. Sometimes there is a 52kDa band on WB; this is not p52. There is a cleavage site on the NFKB2 (Swiss-Prot No.: Q00653). The cleavage site is between 454 and 455 (cleavage when cotranslationally processed). The antibody can interact with the C-half sometimes. The MW. of C-half is near 52Kd.

     This product was previously labelled as NFkB p100

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • NFkB
    • p52
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • NFkB pathway
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • NFkB
    • p52

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Rat
Application Abreviews Notes
WB
1/500 - 1/1000. Predicted molecular weight: 97 kDa.
IHC-P
1/50 - 1/100.
ICC/IF
1/100 - 1/200.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 97 kDa.
IHC-P
1/50 - 1/100.
ICC/IF
1/100 - 1/200.

Target

  • Relevance

    NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

    • Entrez Gene: 4791 Human
    • Entrez Gene: 309452 Rat
    • Omim: 164012 Human
    • SwissProt: Q00653 Human
    • Unigene: 73090 Human
    • Unigene: 204814 Rat
    • Alternative names

      • CVID10 antibody
      • DNA binding factor KBF2 antibody
      • H2TF1 antibody
      • Lymphocyte translocation chromosome 10 protein antibody
      • LYT 10 antibody
      • NF kB2 antibody
      • NFKB p52/p100 subunit antibody
      • Nuclear factor Kappa B subunit 2 antibody
      • Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 (p49/p100) antibody
      • Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 antibody
      • Oncogene Lyt 10 antibody
      • p100 antibody
      • Transcription factor NFKB2 antibody
      see all

    Images

    • Western blot - Anti-NFkB p100/NFKB2 antibody (ab131539)
      Western blot - Anti-NFkB p100/NFKB2 antibody (ab131539)
      All lanes : Anti-NFkB p100/NFKB2 antibody (ab131539) at 1/500 dilution

      Lane 1 : HT29 cell extract with blocking peptide
      Lane 2 : HT29 cell extract

      Predicted band size: 97 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-NFkB p100/NFKB2 antibody (ab131539)
      Immunocytochemistry/ Immunofluorescence - Anti-NFkB p100/NFKB2 antibody (ab131539)

      Immunofluorescence analysis of methanol-fixed HeLa cells labelling NFkB p100/NFKB2 with ab131539 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (ab131539)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (ab131539)

      Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling NFkB p100/NFKB2 with ab131539 at 1/50 dilution. Right panel was preincubated with blocking peptide.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab131539? Please let us know so that we can cite the reference in this datasheet.

    ab131539 has been referenced in 3 publications.

    • Chen Z  et al. Two-layer regulation of TRAF6 mediated by both TLR4/NF-kB signaling and miR-589-5p increases proinflammatory cytokines in the pathology of severe acute pancreatitis. Am J Transl Res 12:2379-2395 (2020). PubMed: 32655778
    • McDonald MK  et al. Regulation of proinflammatory genes by the circulating microRNA hsa-miR-939. Sci Rep 6:30976 (2016). ICC . PubMed: 27498764
    • Xu J  et al. Inhibition of PHLPP2/cyclin D1 protein translation contributes to the tumor suppressive effect of NF?B2 (p100). Oncotarget 7:34112-30 (2016). PubMed: 27095572

    Customer reviews and Q&As

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