Product nameAnti-NFkB p100/NFKB2 antibody
See all NFkB p100/NFKB2 primary antibodies
DescriptionRabbit polyclonal to NFkB p100/NFKB2
SpecificityThe antibody detects endogenous level of total NFkB-p100 protein.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- HeLa cells; HT29 cell extract; Human lung carcinoma tissue
This antibody detects sites on p100 only. Sometimes there is a 52kDa band on WB; this is not p52. There is a cleavage site on the NFKB2 (Swiss-Prot No.: Q00653). The cleavage site is between 454 and 455 (cleavage when cotranslationally processed). The antibody can interact with the C-half sometimes. The MW. of C-half is near 52Kd.
This product was previously labelled as NFkB p100
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab131539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 97 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
RelevanceNF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
Cellular localizationCytoplasmic and Nuclear
- CVID10 antibody
- DNA binding factor KBF2 antibody
- H2TF1 antibody
All lanes : Anti-NFkB p100/NFKB2 antibody (ab131539) at 1/500 dilution
Lane 1 : HT29 cell extract with blocking peptide
Lane 2 : HT29 cell extract
Predicted band size: 97 kDa
Immunofluorescence analysis of methanol-fixed HeLa cells labelling NFkB p100/NFKB2 with ab131539 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling NFkB p100/NFKB2 with ab131539 at 1/50 dilution. Right panel was preincubated with blocking peptide.
This product has been referenced in:
- McDonald MK et al. Regulation of proinflammatory genes by the circulating microRNA hsa-miR-939. Sci Rep 6:30976 (2016). ICC . Read more (PubMed: 27498764) »
- Xu J et al. Inhibition of PHLPP2/cyclin D1 protein translation contributes to the tumor suppressive effect of NF?B2 (p100). Oncotarget 7:34112-30 (2016). Read more (PubMed: 27095572) »