For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    By product type
    Proteins and Peptides
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Lysates
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    • Protocols & troubleshooting
    • Technical FAQs
    • Get technical help
    • RabMAb Advantages
    • Buying FAQs
    • Antibody Guide
    • Scientific webinars
    • Biochemical product FAQ
    • Support resources
    • eProcurement
    Check out our protocols

    Visit protocols and troubleshooting or check them out using the Abcam app for iPhone

    Protocols and troubleshooting

    iPhone app

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    nfkb-p100nfkb2-antibody-c-terminal-ab227096.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Signaling Pathway Nuclear Signaling NFkB Pathway
Share by email
Validated using a knockout cell line

Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)

  • Datasheet
  • SDS
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

  • Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
  • Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
  • Immunoprecipitation - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
  • Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
  • Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
  • Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)

Add compatible products

Primary

Product image

 

Secondary

Product image

 

Unfortunately, one or more of the products below are unavailable in your country/region.

Sorry, we can't display this right now.
Please contact us to place your order, or try again later.

View more associated products

  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-NFkB p100/NFKB2 antibody - C-terminal
    See all NFkB p100/NFKB2 primary antibodies
  • Description

    Rabbit polyclonal to NFkB p100/NFKB2 - C-terminal
  • Host species

    Rabbit
  • Specificity

    ab227096 specifically recognizes the NFkB p100/NFKB2 isoform, but not the cleaved p52 isoform.

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment within Human NFkB p100/NFKB2 (C terminal). The exact sequence is proprietary.
    Database link: Q00653

  • Positive control

    • WB: A431 cell extract; HeLa whole cell extract; HeLa, HepG2 and NIH/3T3 whole cell lysates. IP: Jurkat whole cell lysate.
  • General notes

     This product was previously labelled as NFkB p100

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HepG2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • A431 whole cell lysate (ab30132)
    • NIH 3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)
    • HepG2 whole cell lysate (ab7900)
    • A431 whole cell lysate (ab7909)

Applications

Our Abpromise guarantee covers the use of ab227096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 97 kDa.
IP 1/100 - 1/500.

Target

  • Relevance

    NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

    • Entrez Gene: 4791 Human
    • Entrez Gene: 18034 Mouse
    • Entrez Gene: 309452 Rat
    • Omim: 164012 Human
    • SwissProt: Q00653 Human
    • SwissProt: Q9WTK5 Mouse
    • Unigene: 73090 Human
    • Unigene: 102365 Mouse
    • Unigene: 204814 Rat
    see all
  • Alternative names

    • CVID10 antibody
    • DNA binding factor KBF2 antibody
    • H2TF1 antibody
    • Lymphocyte translocation chromosome 10 protein antibody
    • LYT 10 antibody
    • NF kB2 antibody
    • NFKB p52/p100 subunit antibody
    • Nuclear factor Kappa B subunit 2 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 (p49/p100) antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 antibody
    • Oncogene Lyt 10 antibody
    • p100 antibody
    • Transcription factor NFKB2 antibody
    see all

Images

  • Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    All lanes : Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : NFkB p100/NFKB2 Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 97 kDa

  • Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

    Predicted band size: 97 kDa



    7.5% SDS-PAGE gel.

  • Immunoprecipitation - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    Immunoprecipitation - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)

    NFkB p100/NFKB2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) with 2 µg ab227096. Western blot was performed from the immunoprecipitate using ab227096. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Jurkat whole cell lysate 30 μg.

    Lane 2: Control IP in Jurkat whole cell lysate using 2 μg of preimmune rabbit IgG.

    Lane 3: ab227096 IP in Jurkat whole cell lysate.

     

  • Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    All lanes : Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/500 dilution

    Lane 1 : Wild type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : NFkB p100/NFKB2 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 97 kDa



    7.5% SDS-PAGE gel.

  • Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    All lanes : Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 97 kDa



    7.5% SDS-PAGE gel.

  • Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Predicted band size: 97 kDa



    7.5% SDS-PAGE gel.

Datasheets and documents

    • Datasheet
    • SDS
  • References

    ab227096 has not yet been referenced specifically in any publications.

    Publishing research using ab227096? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab227096.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & advice
    • Buying FAQs
    • RabMAb products
    • Biochemical product FAQs
    Company
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2019 Abcam plc. All rights reserved.