
Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-NFkB p100/NFKB2 antibody - C-terminal
See all NFkB p100/NFKB2 primary antibodies -
Description
Rabbit polyclonal to NFkB p100/NFKB2 - C-terminal -
Host species
Rabbit -
Specificity
ab227096 specifically recognizes the NFkB p100/NFKB2 isoform, but not the cleaved p52 isoform.
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Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment within Human NFkB p100/NFKB2 (C terminal). The exact sequence is proprietary.
Database link: Q00653 -
Positive control
- WB: A431 cell extract; HeLa whole cell extract; HeLa, HepG2 and NIH/3T3 whole cell lysates. IP: Jurkat whole cell lysate.
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General notes
This product was previously labelled as NFkB p100
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- A431 whole cell lysate (ab30132)
- NIH 3T3 whole cell lysate (ab7179)
- Jurkat whole cell lysate (ab7899)
- HepG2 whole cell lysate (ab7900)
- A431 whole cell lysate (ab7909)
Applications
Our Abpromise guarantee covers the use of ab227096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/500 - 1/3000. Predicted molecular weight: 97 kDa. | |
IP | 1/100 - 1/500. |
Target
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Relevance
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 4791 Human
- Entrez Gene: 18034 Mouse
- Entrez Gene: 309452 Rat
- Omim: 164012 Human
- SwissProt: Q00653 Human
- SwissProt: Q9WTK5 Mouse
- Unigene: 73090 Human
- Unigene: 102365 Mouse
see all -
Alternative names
- CVID10 antibody
- DNA binding factor KBF2 antibody
- H2TF1 antibody
see all
Images
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All lanes : Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : NFkB p100/NFKB2 Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 97 kDa -
Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg
Predicted band size: 97 kDa7.5% SDS-PAGE gel.
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NFkB p100/NFKB2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) with 2 µg ab227096. Western blot was performed from the immunoprecipitate using ab227096. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Jurkat whole cell lysate 30 μg.
Lane 2: Control IP in Jurkat whole cell lysate using 2 μg of preimmune rabbit IgG.
Lane 3: ab227096 IP in Jurkat whole cell lysate.
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All lanes : Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/500 dilution
Lane 1 : Wild type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : NFkB p100/NFKB2 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 97 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 97 kDa7.5% SDS-PAGE gel.
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Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 97 kDa7.5% SDS-PAGE gel.
References
ab227096 has not yet been referenced specifically in any publications.