Overview

  • Product name

    Anti-NFkB p105 / p50 (phospho S337) antibody
    See all NFkB p105 / p50 primary antibodies
  • Description

    Rabbit polyclonal to NFkB p105 / p50 (phospho S337)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NFkB p105/ p50 (phospho S337). The exact sequence is proprietary. Amino acid sequence corresponds to residues surrounding S337.
    Database link: P19838

  • Positive control

    • HeLa cells, Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab194729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 105 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
  • Sequence similarities

    Contains 7 ANK repeats.
    Contains 1 death domain.
    Contains 1 RHD (Rel-like) domain.
  • Domain

    The C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding, and transcription activation.
    Glycine-rich region (GRR) appears to be a critical element in the generation of p50.
  • Post-translational
    modifications

    While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p50 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing.
    Phosphorylation at 'Ser-903' and 'Ser-907' primes p105 for proteolytic processing in response to TNF-alpha stimulation. Phosphorylation at 'Ser-927' and 'Ser-932' are required for BTRC/BTRCP-mediated proteolysis.
    Polyubiquitination seems to allow p105 processing.
    S-nitrosylation of Cys-61 affects DNA binding.
  • Cellular localization

    Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686C01211 antibody
    • DNA binding factor KBF1 antibody
    • DNA binding factor KBF1 EBP1 antibody
    • DNA-binding factor KBF1 antibody
    • EBP 1 antibody
    • EBP-1 antibody
    • EBP1 antibody
    • KBF1 antibody
    • MGC54151 antibody
    • nf b antibody
    • NF kappa B antibody
    • NF kappaB antibody
    • NF kappabeta antibody
    • NF kB1 antibody
    • NF-kappaB antibody
    • NFkappaB antibody
    • NFKB 1 antibody
    • NFKB p105 antibody
    • NFKB p50 antibody
    • NFKB-p105 antibody
    • Nfkb1 antibody
    • NFKB1_HUMAN antibody
    • Nuclear factor kappa B DNA binding subunit antibody
    • Nuclear factor kappa-B antibody
    • Nuclear factor kappa-B, subunit 1 antibody
    • Nuclear factor NF kappa B p105 subunit antibody
    • Nuclear factor NF kappa B p50 subunit antibody
    • Nuclear factor NF-kappa-B p50 subunit antibody
    • Nuclear factor of kappa light chain gene enhancer in B cells 1 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 antibody
    • p105 antibody
    • p50 antibody
    • p84/NF-kappa-B1 p98 antibody
    • Transcription factor NFKB1 antibody
    see all

Images

  • Anti-NFkB p105 / p50 (phospho S337) antibody (ab194729) at 1/500 dilution + Extract of HeLa cells

    Predicted band size: 105 kDa

  • Immunofluorescent analysis of methanol fixed HeLa cells labeling NFkB p105 / p50 (phospho S337) with ab194729 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling NFkB p105 / p50 (phospho S337) with ab194729 at 1/50.

References

ab194729 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Healthy Rat Brain)
Antigen retrieval step
None
Permeabilization
Yes - Triton X100 0.3%
Specification
Healthy Rat Brain
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde

Antoine Presset

Verified customer

Submitted Apr 24 2019

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