Key features and details
- Rabbit polyclonal to NFKBID
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NFKBID antibody
See all NFKBID primary antibodies
DescriptionRabbit polyclonal to NFKBID
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment (His-T7-tag) corresponding to Human NFKBID aa 46-285. (Expressed in E.coli).
DEEGDTLLHLFAARGLRWAAYAAAEVLQVYRRLDIREHKGKTPLLVAAAA NQPLIVEDLLNLGAEPNAADHQGRSVLHVAATYGLPGVLLAVLNSGVQVD LEARDFEGLTPLHTAILALNVAMRPSDLCPRVLSTQARDRLDCVHMLLQM GANHTSQEIKSNKTVLHLAVQAANPTLVQLLLELPRGDLRTFVNMKAHGN TALHMAAALPPGPAQEAIVRHLLAAGADPTLRNLENEQPV
Database link: Q8NI38
- IHC-P: Human liver and glioma tissues. WB: Recombinant human NFKBID protein; Human serum; Human liver lysate; Mouse kidney lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232913 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 33 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionMay regulate the expression of IL-2, IL-6, and other cytokines through regulation on NF-kappa-B activity. Functions in the regulation of inflammatory responses. May also regulate TCR-induced negative selection of thymocytes.
Sequence similaritiesBelongs to the NF-kappa-B inhibitor family.
Contains 6 ANK repeats.
- Information by UniProt
- I-kappa-B-delta antibody
- IkappaBdelta antibody
- IkappaBNS antibody
All lanes : Anti-NFKBID antibody (ab232913) at 3 µg/ml
Lane 1 : Human serum
Lane 2 : Human liver lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Human lung lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 33 kDa
Anti-NFKBID antibody (ab232913) at 5 µg/ml + Recombinant human NFKBID protein
Predicted band size: 33 kDa
Paraffin-embedded human glioma tissue stained for NFKBID using ab232913 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human liver tissue stained for NFKBID using ab232913 at 20 µg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232913 has not yet been referenced specifically in any publications.