Product nameAnti-NFKBIL2 antibody
See all NFKBIL2 primary antibodies
DescriptionRabbit polyclonal to NFKBIL2
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Leaf frog
Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human NFKBIL2.
- This antibody gave a positive signal in HeLa whole cell lysate and HeLa nuclear lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab101898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200. Use paraformaldehyde fixed cells, permeabilized with 0.5% Triton X-100/PBS.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 175 kDa (predicted molecular weight: 151 kDa).|
FunctionComponent of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold.
Tissue specificityExpressed in heart, skeletal muscle and tracheal epithelial cells.
Sequence similaritiesBelongs to the Tonsoku family.
Contains 3 ANK repeats.
Contains 7 LRR (leucine-rich) repeats.
Contains 8 TPR repeats.
DomainThe ANK repeats mediate the interaction with the MCM complex and histones, while the LRR repeats mediate the interaction with MMS22L.
Cellular localizationCytoplasm. Nucleus. Mainly nuclear. Localizes to DNA damage sites, accumulates at stressed replication forks.
- Information by UniProt
- I kappa B related protein antibody
- I-kappa-B-related protein antibody
- IkappaBR antibody
All lanes : Anti-NFKBIL2 antibody (ab101898) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 151 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
Additional bands at: 43 kDa, 67 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Ab101898 detects a band at approximately 170-kDa. While this differs to its predicted molecular weight of 151-kDa, this band has been observed in the literature (PMID:21055983).
ab101898 (1/200) staining NFKBIL2 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.