This antibody gave a positive signal in HeLa whole cell lysate and HeLa nuclear lysate.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200. Use paraformaldehyde fixed cells, permeabilized with 0.5% Triton X-100/PBS.
Use a concentration of 1 µg/ml. Detects a band of approximately 175 kDa (predicted molecular weight: 151 kDa).
Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold.
Expressed in heart, skeletal muscle and tracheal epithelial cells.
Belongs to the Tonsoku family. Contains 3 ANK repeats. Contains 7 LRR (leucine-rich) repeats. Contains 8 TPR repeats.
The ANK repeats mediate the interaction with the MCM complex and histones, while the LRR repeats mediate the interaction with MMS22L.
Cytoplasm. Nucleus. Mainly nuclear. Localizes to DNA damage sites, accumulates at stressed replication forks.
Ab101898 detects a band at approximately 170-kDa. While this differs to its predicted molecular weight of 151-kDa, this band has been observed in the literature (PMID:21055983).
Immunocytochemistry/ Immunofluorescence - Anti-NFKBIL2 antibody (ab101898)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
ab101898 (1/200) staining NFKBIL2 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.