• Product name

  • Description

    Rabbit polyclonal to NFKBIL2
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Leaf frog
  • Immunogen

    Synthetic peptide corresponding to Human NFKBIL2 aa 500-600 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab117409)

  • Positive control

    • This antibody gave a positive signal in HeLa whole cell lysate and HeLa nuclear lysate.



Our Abpromise guarantee covers the use of ab101898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200. Use paraformaldehyde fixed cells, permeabilized with 0.5% Triton X-100/PBS.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 175 kDa (predicted molecular weight: 151 kDa).


  • Function

    Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold.
  • Tissue specificity

    Expressed in heart, skeletal muscle and tracheal epithelial cells.
  • Sequence similarities

    Belongs to the Tonsoku family.
    Contains 3 ANK repeats.
    Contains 7 LRR (leucine-rich) repeats.
    Contains 8 TPR repeats.
  • Domain

    The ANK repeats mediate the interaction with the MCM complex and histones, while the LRR repeats mediate the interaction with MMS22L.
  • Cellular localization

    Cytoplasm. Nucleus. Mainly nuclear. Localizes to DNA damage sites, accumulates at stressed replication forks.
  • Information by UniProt
  • Database links

  • Alternative names

    • I kappa B related protein antibody
    • I-kappa-B-related protein antibody
    • IkappaBR antibody
    • IKBR antibody
    • Inhibitor of kappa B related protein antibody
    • Inhibitor of kappa B-related protein antibody
    • MMS22L-interacting protein antibody
    • MMS22L-interacting protein, NFBIL2/TONSL antibody
    • NF kappa B inhibitor like protein 2 antibody
    • NF-kappa-B inhibitor-like protein 2 antibody
    • NFBIL2 antibody
    • NFBIL2/TONSL antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor like protein 2 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2 antibody
    • Protein IkappaBR antibody
    • Tonsl antibody
    • TONSL_HUMAN antibody
    • Tonsoku-like protein antibody
    • tonsoku-like, DNA repair protein antibody
    see all


  • All lanes : Anti-NFKBIL2 antibody (ab101898) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 151 kDa
    Observed band size: 175 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 43 kDa, 67 kDa, 80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 20 minutes

    Ab101898 detects a band at approximately 170-kDa. While this differs to its predicted molecular weight of 151-kDa, this band has been observed in the literature (PMID:21055983).
  • ab101898 (1/200) staining NFKBIL2 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

    See Abreview


This product has been referenced in:

  • Saredi G  et al. H4K20me0 marks post-replicative chromatin and recruits the TONSL–MMS22L DNA repair complex. Nature 534:714-718 (2016). WB . Read more (PubMed: 27338793) »
See 1 Publication for this product

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa)
Yes - 0.5% Triton-X100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted Nov 15 2011

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