Anti-NFX1 antibody (ab176733)
Key features and details
- Rabbit polyclonal to NFX1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NFX1 antibody -
Description
Rabbit polyclonal to NFX1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human NFX1 aa 1070-1120. The exact sequence is proprietary. NP_002495.2.
Database link: Q12986 -
Positive control
- HeLa, 293T and NIH3T3 whole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-5 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
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Notes |
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IP
Use at 2-5 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 124 kDa. |
Target
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Relevance
NFX1 is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. It may play a role in the inflammatory response, regulating its duration by limiting the period in which class II MHC molecules are induced by IFN gamma. The RING type zinc finger domain interacts with an ubiquitin conjugating enzyme (E2) and facilitates ubiquitination. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 4799 Human
- Entrez Gene: 74164 Mouse
- Omim: 603255 Human
- SwissProt: Q12986 Human
- SwissProt: B1AY10 Mouse
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Alternative names
- MGC20369 antibody
- NFX 1 antibody
- NFX1 antibody
see all
Images
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All lanes : Anti-NFX1 antibody (ab176733) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 30 seconds -
ab176733 at 0.4 µg/ml detecting NFX1 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of NFX1.
Lane 2: IP with an antibody which recognizes an upstream epitope of NFX1.Lane 3: ab176733 at 3 µg/mg of lysate
Lane 4: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab176733 has been referenced in 1 publication.
- Wu P et al. Tumor Cell-Derived TGFß1 Attenuates Antitumor Immune Activity of T Cells via Regulation of PD-1 mRNA. Cancer Immunol Res 8:1470-1484 (2020). PubMed: 32999004