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Synthetic peptide within Human NFX1 aa 1070-1120. The exact sequence is proprietary. NP_002495.2.
Database link: Q12986
Our Abpromise guarantee covers the use of ab176733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 124 kDa.|
ab176733 at 0.4 µg/ml detecting NFX1 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of NFX1.
Lane 2: IP with an antibody which recognizes an upstream epitope of NFX1.
Lane 3: ab176733 at 3 µg/mg of lysate
Lane 4: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab176733 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"