Overview

  • Product name
  • Description
    Rabbit polyclonal to NFX1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human NFX1 aa 1070-1120. The exact sequence is proprietary. NP_002495.2.
    Database link: Q12986

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab176733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 124 kDa.

Target

  • Relevance
    NFX1 is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. It may play a role in the inflammatory response, regulating its duration by limiting the period in which class II MHC molecules are induced by IFN gamma. The RING type zinc finger domain interacts with an ubiquitin conjugating enzyme (E2) and facilitates ubiquitination.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • MGC20369 antibody
    • NFX 1 antibody
    • NFX1 antibody
    • NFX2 antibody
    • Nuclear transcription factor X box binding 1 antibody
    • Nuclear transcription factor X box binding protein 1 antibody
    • Transcriptional repressor NF X1 antibody
    see all

Images

  • All lanes : Anti-NFX1 antibody (ab176733) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 124 kDa


    Exposure time: 30 seconds
  • ab176733 at 0.4 µg/ml detecting NFX1 in HeLa whole cell lysate by WB following IP.

    Lane 1: IP with an antibody which recognizes an upstream epitope of NFX1.
    Lane 2: IP with an antibody which recognizes an upstream epitope of NFX1.

    Lane 3: ab176733 at 3 µg/mg of lysate

    Lane 4: Control IgG.

    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab176733 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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