Anti-NFXL1 antibody (ab242121)
Key features and details
- Rabbit polyclonal to NFXL1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NFXL1 antibody
See all NFXL1 primary antibodies -
Description
Rabbit polyclonal to NFXL1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NFXL1 aa 600-650. The exact sequence is proprietary. NP_694540.3
Database link: Q6ZNB6 -
Positive control
- WB: HeLa, Jurkat and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to NFXL1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab242121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 101 kDa. |
Target
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Sequence similarities
Belongs to the NFX1 family.
Contains 10 NF-X1-type zinc fingers.
Contains 1 RING-type zinc finger. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 152518 Human
- SwissProt: Q6ZNB6 Human
- Unigene: 646325 Human
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Alternative names
- hOZFP antibody
- NF-X1-type zinc finger protein NFXL1 antibody
- NFXL1 antibody
see all
Images
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Western blot - Anti-NFXL1 antibody (ab242121)All lanes : Anti-NFXL1 antibody (ab242121) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 101 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-NFXL1 antibody (ab242121)NFXL1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab242121 used for IP at 6 µg/mg lysate. For WB at 0.4 µg/ml.
Lane 1: ab242121 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Chemiluminescence detection: 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab242121 has not yet been referenced specifically in any publications.
