Anti-NFXL1 antibody (ab242121)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-NFXL1 antibody
See all NFXL1 primary antibodies -
Description
Rabbit polyclonal to NFXL1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NFXL1 aa 600-650. The exact sequence is proprietary. NP_694540.3
Database link: Q6ZNB6 -
Positive control
- WB: HeLa, Jurkat and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to NFXL1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab242121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 101 kDa. |
Target
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Sequence similarities
Belongs to the NFX1 family.
Contains 10 NF-X1-type zinc fingers.
Contains 1 RING-type zinc finger. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 152518 Human
- SwissProt: Q6ZNB6 Human
- Unigene: 646325 Human
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Alternative names
- hOZFP antibody
- NF-X1-type zinc finger protein NFXL1 antibody
- NFXL1 antibody
see all
Images
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Western blot - Anti-NFXL1 antibody (ab242121)All lanes : Anti-NFXL1 antibody (ab242121) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 101 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-NFXL1 antibody (ab242121)NFXL1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab242121 used for IP at 6 µg/mg lysate. For WB at 0.4 µg/ml.
Lane 1: ab242121 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Chemiluminescence detection: 30 seconds.
Datasheets and documents
References
ab242121 has not yet been referenced specifically in any publications.
