Product nameAnti-NFXL1 antibody
See all NFXL1 primary antibodies
DescriptionRabbit polyclonal to NFXL1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human NFXL1 aa 600-650. The exact sequence is proprietary. NP_694540.3
Database link: Q6ZNB6
- WB: HeLa, Jurkat and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to NFXL1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab242121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 101 kDa.|
Sequence similaritiesBelongs to the NFX1 family.
Contains 10 NF-X1-type zinc fingers.
Contains 1 RING-type zinc finger.
- Information by UniProt
- hOZFP antibody
- NF-X1-type zinc finger protein NFXL1 antibody
- NFXL1 antibody
All lanes : Anti-NFXL1 antibody (ab242121) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 101 kDa
Exposure time: 3 minutes
NFXL1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab242121 used for IP at 6 µg/mg lysate. For WB at 0.4 µg/ml.
Lane 1: ab242121 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Chemiluminescence detection: 30 seconds.
ab242121 has not yet been referenced specifically in any publications.