Anti-NFYA antibody (ab154554)
Key features and details
- Rabbit polyclonal to NFYA
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NFYA antibody
See all NFYA primary antibodies -
Description
Rabbit polyclonal to NFYA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 and 347 of Human NFYA.
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Positive control
- A431, HeLa, HepG2, NIH 3T3, JC and BCL1 whole cell lysates; HeLa cells; D54 xenograft
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 37 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. -
Sequence similarities
Belongs to the NFYA/HAP2 subunit family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4800 Human
- Entrez Gene: 18044 Mouse
- Omim: 189903 Human
- SwissProt: P23511 Human
- SwissProt: P23708 Mouse
- Unigene: 10441 Human
- Unigene: 4929 Mouse
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Alternative names
- CAAT box DNA binding protein subunit A antibody
- CAAT box DNA-binding protein subunit A antibody
- CBF A antibody
see all
Images
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All lanes : Anti-NFYA antibody (ab154554) at 1/3000 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
10% SDS PAGE -
All lanes : Anti-NFYA antibody (ab154554) at 1/3000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin embedded Human D54 xenograft labeling NFYA with ab154554 at 1/500 dilution.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa cell labeling NFYA with ab154554 at 1/500 dilution. The image in the lower panel was costained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154554 has not yet been referenced specifically in any publications.