Anti-NFYA antibody (ab154554)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-NFYA antibody
See all NFYA primary antibodies -
Description
Rabbit polyclonal to NFYA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 and 347 of Human NFYA.
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Positive control
- A431, HeLa, HepG2, NIH 3T3, JC and BCL1 whole cell lysates; HeLa cells; D54 xenograft
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 37 kDa. | |
| IHC-P | 1/100 - 1/1000. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. -
Sequence similarities
Belongs to the NFYA/HAP2 subunit family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4800 Human
- Entrez Gene: 18044 Mouse
- Omim: 189903 Human
- SwissProt: P23511 Human
- SwissProt: P23708 Mouse
- Unigene: 10441 Human
- Unigene: 4929 Mouse
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Alternative names
- CAAT box DNA binding protein subunit A antibody
- CAAT box DNA-binding protein subunit A antibody
- CBF A antibody
see all
Images
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Western blot - Anti-NFYA antibody (ab154554)All lanes : Anti-NFYA antibody (ab154554) at 1/3000 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
10% SDS PAGE -
Western blot - Anti-NFYA antibody (ab154554)All lanes : Anti-NFYA antibody (ab154554) at 1/3000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA antibody (ab154554)Immunohistochemical analysis of paraffin embedded Human D54 xenograft labeling NFYA with ab154554 at 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody (ab154554)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cell labeling NFYA with ab154554 at 1/500 dilution. The image in the lower panel was costained with Hoechst 33342.
Protocols
References
ab154554 has not yet been referenced specifically in any publications.
