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    nfya-antibody-ab154554.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-NFYA antibody (ab154554)

  • Datasheet
  • SDS
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Western blot - Anti-NFYA antibody (ab154554)
  • Western blot - Anti-NFYA antibody (ab154554)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA antibody (ab154554)
  • Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody (ab154554)

Key features and details

  • Rabbit polyclonal to NFYA
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-NFYA antibody
    See all NFYA primary antibodies
  • Description

    Rabbit polyclonal to NFYA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 347 of Human NFYA.

  • Positive control

    • A431, HeLa, HepG2, NIH 3T3, JC and BCL1 whole cell lysates; HeLa cells; D54 xenograft
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • NIH 3T3 whole cell lysate (ab7179)
    • A431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human NFYA protein (ab152040)
    • Recombinant Human NFYA protein (ab192274)

Applications

Our Abpromise guarantee covers the use of ab154554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
  • Sequence similarities

    Belongs to the NFYA/HAP2 subunit family.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P23511 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4800 Human
    • Entrez Gene: 18044 Mouse
    • Omim: 189903 Human
    • SwissProt: P23511 Human
    • SwissProt: P23708 Mouse
    • Unigene: 10441 Human
    • Unigene: 4929 Mouse
    • Alternative names

      • CAAT box DNA binding protein subunit A antibody
      • CAAT box DNA-binding protein subunit A antibody
      • CBF A antibody
      • CBF B antibody
      • CBFA antibody
      • CBFB antibody
      • CCAAT binding transcription factor subunit B antibody
      • FLJ11236 antibody
      • HAP 2 antibody
      • HAP2 antibody
      • HAP2 CCAAT binding protein antibody
      • NF YA antibody
      • NF-YA antibody
      • NFY protein chain A antibody
      • NFYA antibody
      • NFYA_HUMAN antibody
      • NUCLEAR FACTOR BINDING TO Y BOX OF HLA GENES antibody
      • Nuclear transcription factor Y alpha antibody
      • Nuclear transcription factor Y antibody
      • Nuclear transcription factor Y subunit A antibody
      • Nuclear transcription factor Y subunit alpha antibody
      • Sez10 antibody
      • Transcription factor NF Y A subunit antibody
      see all

    Images

    • Western blot - Anti-NFYA antibody (ab154554)
      Western blot - Anti-NFYA antibody (ab154554)
      All lanes : Anti-NFYA antibody (ab154554) at 1/3000 dilution

      Lane 1 : NIH 3T3 whole cell lysate
      Lane 2 : JC whole cell lysate
      Lane 3 : BCL1 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 37 kDa



      10% SDS PAGE
    • Western blot - Anti-NFYA antibody (ab154554)
      Western blot - Anti-NFYA antibody (ab154554)
      All lanes : Anti-NFYA antibody (ab154554) at 1/3000 dilution

      Lane 1 : A431 whole cell lysate
      Lane 2 : HeLa whole cell lysate
      Lane 3 : HepG2 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 37 kDa



      10% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA antibody (ab154554)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA antibody (ab154554)
      Immunohistochemical analysis of paraffin embedded Human D54 xenograft labeling NFYA with ab154554 at 1/500 dilution.
    • Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody (ab154554)
      Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody (ab154554)
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa cell labeling NFYA with ab154554 at 1/500 dilution. The image in the lower panel was costained with Hoechst 33342.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab154554? Please let us know so that we can cite the reference in this datasheet.

    ab154554 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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