Overview

  • Product name
    Anti-NFYA antibody
    See all NFYA primary antibodies
  • Description
    Rabbit polyclonal to NFYA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 347 of Human NFYA.

  • Positive control
    • A431, HeLa, HepG2, NIH 3T3, JC and BCL1 whole cell lysates; HeLa cells; D54 xenograft

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
  • Sequence similarities
    Belongs to the NFYA/HAP2 subunit family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • CAAT box DNA binding protein subunit A antibody
      • CAAT box DNA-binding protein subunit A antibody
      • CBF A antibody
      • CBF B antibody
      • CBFA antibody
      • CBFB antibody
      • CCAAT binding transcription factor subunit B antibody
      • FLJ11236 antibody
      • HAP 2 antibody
      • HAP2 antibody
      • HAP2 CCAAT binding protein antibody
      • NF YA antibody
      • NF-YA antibody
      • NFY protein chain A antibody
      • NFYA antibody
      • NFYA_HUMAN antibody
      • NUCLEAR FACTOR BINDING TO Y BOX OF HLA GENES antibody
      • Nuclear transcription factor Y alpha antibody
      • Nuclear transcription factor Y antibody
      • Nuclear transcription factor Y subunit A antibody
      • Nuclear transcription factor Y subunit alpha antibody
      • Sez10 antibody
      • Transcription factor NF Y A subunit antibody
      see all

    Images

    • Western blot - Anti-NFYA antibody (ab154554)
      Western blot - Anti-NFYA antibody (ab154554)
      All lanes : Anti-NFYA antibody (ab154554) at 1/3000 dilution

      Lane 1 : NIH 3T3 whole cell lysate
      Lane 2 : JC whole cell lysate
      Lane 3 : BCL1 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 37 kDa



      10% SDS PAGE
    • Western blot - Anti-NFYA antibody (ab154554)
      Western blot - Anti-NFYA antibody (ab154554)
      All lanes : Anti-NFYA antibody (ab154554) at 1/3000 dilution

      Lane 1 : A431 whole cell lysate
      Lane 2 : HeLa whole cell lysate
      Lane 3 : HepG2 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 37 kDa



      10% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA antibody (ab154554)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA antibody (ab154554)
      Immunohistochemical analysis of paraffin embedded Human D54 xenograft labeling NFYA with ab154554 at 1/500 dilution.
    • Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody (ab154554)
      Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody (ab154554)
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa cell labeling NFYA with ab154554 at 1/500 dilution. The image in the lower panel was costained with Hoechst 33342.

    References

    ab154554 has not yet been referenced specifically in any publications.

    Publishing research using ab154554? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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