Product nameAnti-NFYA antibody
See all NFYA primary antibodies
DescriptionRabbit polyclonal to NFYA
Tested applicationsSuitable for: IP, IHC-Pmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Synthetic peptide within Human NFYA aa 320-333. The exact sequence is proprietary. NP_002496.1
Database link: P23511
- IHC-P: Human non-small cell lung cancer tissue; IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
Our Abpromise guarantee covers the use of ab264274 in the following tested applications.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
FunctionStimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
Sequence similaritiesBelongs to the NFYA/HAP2 subunit family.
- Information by UniProt
- CAAT box DNA binding protein subunit A antibody
- CAAT box DNA-binding protein subunit A antibody
- CBF A antibody
NFYA was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264274 at 3 μg per reaction. Western blot was performed on the immunoprecipitates using a different NFYA antibody.
Lane 1: ab264274 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.
Exposure time: 5 seconds.
Immunohistochemical analysis of FFPE human non-small cell lung cancer tissue section labelling NFYA with ab264274 at 1/1000 dilution. Detection: DAB.
ab264274 has not yet been referenced specifically in any publications.