Product nameAnti-NFYA antibody
See all NFYA primary antibodies
DescriptionRabbit polyclonal to NFYA
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Synthetic peptide within Human NFYA aa 333-347. The exact sequence is proprietary. NP_002496.1
Database link: P23511
- IHC-P: Human breast carcinoma tissue; IP: HeLa whole cell lysate; WB: HeLa, Hek-293T and NIH-3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab264275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 37 kDa.|
FunctionStimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
Sequence similaritiesBelongs to the NFYA/HAP2 subunit family.
- Information by UniProt
- CAAT box DNA binding protein subunit A antibody
- CAAT box DNA-binding protein subunit A antibody
- CBF A antibody
All lanes : Anti-NFYA antibody (ab264275) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Lane 5 : NIH-3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 30 seconds
Immunohistochemical analysis of FFPE human breast carcinoma tissue section (left) and FFPE mouse hybridome tumour tissue section (right) labelling NFYA with ab264275 at 1/1000 dilution. Detection: DAB.
NFYA was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264275 at 30μg per reaction. Western blot was performed on the immunoprecipitates using ab264275 at 1 μg/ml.
Lane 1: ab264275 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.
Exposure time:5 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264275 has not yet been referenced specifically in any publications.