Recombinant
RabMAb

Recombinant Anti-NFYA antibody [EPR9061] (ab139402)

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Overview

  • Product name

    Anti-NFYA antibody [EPR9061]
    See all NFYA primary antibodies

  • Description

    Rabbit monoclonal [EPR9061] to NFYA

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human NFYA aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Recombinant Human NFYA protein (ab131777) can be used as a positive control in WB. NIH 3T3, HeLa, 293T, and Jurkat cell lysates; NIH 3T3 cells. This antibody gave a positive result when used in the following methanol fixed cell lines: Malme-3M

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9061

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab139402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.

    • Sequence similarities

      Belongs to the NFYA/HAP2 subunit family.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • CAAT box DNA binding protein subunit A antibody
      • CAAT box DNA-binding protein subunit A antibody
      • CBF A antibody
      • CBF B antibody
      • CBFA antibody
      • CBFB antibody
      • CCAAT binding transcription factor subunit B antibody
      • FLJ11236 antibody
      • HAP 2 antibody
      • HAP2 antibody
      • HAP2 CCAAT binding protein antibody
      • NF YA antibody
      • NF-YA antibody
      • NFY protein chain A antibody
      • NFYA antibody
      • NFYA_HUMAN antibody
      • NUCLEAR FACTOR BINDING TO Y BOX OF HLA GENES antibody
      • Nuclear transcription factor Y alpha antibody
      • Nuclear transcription factor Y antibody
      • Nuclear transcription factor Y subunit A antibody
      • Nuclear transcription factor Y subunit alpha antibody
      • Sez10 antibody
      • Transcription factor NF Y A subunit antibody
      see all

    Images

    • Western blot - Anti-NFYA antibody [EPR9061] (ab139402)
      Western blot - Anti-NFYA antibody [EPR9061] (ab139402)
      All lanes : Anti-NFYA antibody [EPR9061] (ab139402) at 1/1000 dilution

      Lane 1 : NIH 3T3 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 37 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody [EPR9061] (ab139402)
      Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody [EPR9061] (ab139402)

      ab139402 stained Malme-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab139402 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Flow Cytometry - Anti-NFYA antibody [EPR9061] (ab139402)
      Flow Cytometry - Anti-NFYA antibody [EPR9061] (ab139402)
      Flow Cytometrical analysis of permeabilized NIH 3T3 cells labelling NFYA with ab139402 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    ab139402 has not yet been referenced specifically in any publications.

    Publishing research using ab139402? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    ChIP abreview for Anti-NFYA antibody [EPR9061]

     Excellent
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (Prostate cancer cells (PC-3))
    Negative control
    IgG
    Specification
    Prostate cancer cells (PC-3)
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: FORMALDEHYDE
    Positive control
    H3 Histone
    Read More

    Mr. Jacob Elmer

    Verified customer

    Submitted Nov 09 2017

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