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Mouse monoclonal [NFYBA7] to NFYB
Predicted to work with:
Recombinant full length protein (Internal sequence).
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Protein G purified
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Predicted molecular weight: 23 kDa.
Use at an assay dependent dilution.
Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
Belongs to the NFYB/HAP3 subunit family.
Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.
Information by UniProt
CAAT box DNA binding protein subunit B antibody
CAAT box DNA-binding protein subunit B antibody
CBF A antibody
Western blot - NFYB antibody [NFYBA7] (ab51353)
Anti-NFYB antibody [NFYBA7] (ab51353) + immunized recombinant protein
Predicted band size:
Observed band size:
38 kDa (
why is the actual band size different from the predicted?
Additional bands at:
28 kDa, 30 kDa. We are unsure as to the identity of these extra bands.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
SDS-PAGE - Anti-NFYB antibody [NFYBA7] (ab51353)
CBB staining of imunized recombinant protein.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"