Key features and details
- Mouse monoclonal [132.38] to NG2
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG1
Product nameAnti-NG2 antibody [132.38]
See all NG2 primary antibodies
DescriptionMouse monoclonal [132.38] to NG2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat
Does not react with: Mouse
Tissue, cells or virus corresponding to Rat NG2.
- WB: Rat brain, spinal cord and hippocampus tissue lysate.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact email@example.com.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
Primary antibody notesThis antibody is derived from the hybridoma 132.38 produced by the fusion of mouse myeloma cells (P3XAg8.653) and splenocytes from BALB/c mice.
Our Abpromise guarantee covers the use of ab50009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 251 kDa (predicted molecular weight: 251 kDa).
Abcam recommends using BSA as the blocking agent.
FunctionProteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Tissue specificityDetected only in malignant melanoma cells.
Sequence similaritiesContains 15 CSPG (NG2) repeats.
Contains 2 laminin G-like domains.
modificationsO-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
Cellular localizationApical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
- Information by UniProt
- 4732461B14Rik antibody
- AN2 antibody
- AN2 proteoglycan antibody
All lanes : Anti-NG2 antibody [132.38] (ab50009) at 1 µg/ml
Lane 1 : Rat Brain Tissue Lysate
Lane 2 : Rat Spinal Cord Tissue Lysate
Lane 3 : Rat Hippocampus Tissue Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 251 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa (possible non-specific binding), 41 kDa (possible non-specific binding)
Exposure time: 20 minutes
ab50009 has been referenced in 31 publications.
- Santini MP et al. Tissue-Resident PDGFRa+ Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent Manner. Cell Rep 30:555-570.e7 (2020). PubMed: 31940496
- Zhai R et al. Exendin-4, a GLP-1 receptor agonist regulates retinal capillary tone and restores microvascular patency after ischaemia-reperfusion injury. Br J Pharmacol 177:3389-3402 (2020). PubMed: 32232832
- Xi K et al. Microenvironment-responsive immunoregulatory electrospun fibers for promoting nerve function recovery. Nat Commun 11:4504 (2020). PubMed: 32908131
- Zhan Y et al. An MRI Study of Neurovascular Restorative After Combination Treatment With Xiaoshuan Enteric-Coated Capsule and Enriched Environment in Rats After Stroke. Front Neurosci 13:701 (2019). PubMed: 31354412
- Li H et al. Role of TGF-Beta1/SMAD2/3 Pathway in Retinal Outer Deep Vascular Plexus and Photoreceptor Damage in Rat 50/10 Oxygen-Induced Retinopathy. Biomed Res Int 2019:4072319 (2019). PubMed: 31240212