Product nameAnti-NG2 antibody [132.38]
See all NG2 primary antibodies
DescriptionMouse monoclonal [132.38] to NG2
Tested applicationsSuitable for: WB, IP, ELISA, ICC/IF, IHC-Fr, IHC-FoFrmore details
Species reactivityReacts with: Rat
Does not react with: Mouse
Cell extract of HEK293 cells expressing the D3 domain of rat NG2, corresponding to amino acids 1592-2222.
- This antibody gave a positive signal in the following rat tissue lysates: spinal cord; whole brain; hippocampus.
This antibody clone is manufactured by Abcam.
The Protocols tab contains a Mouse on Mouse staining protocol with recommendations when using a mouse monoclonal antibody to stain mouse tissues and tips for reducing background.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
Primary antibody notesThis antibody is derived from the hybridoma 132.38 produced by the fusion of mouse myeloma cells (P3XAg8.653) and splenocytes from BALB/c mice.
Our Abpromise guarantee covers the use of ab50009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 251 kDa (predicted molecular weight: 251 kDa).
Abcam recommends using BSA as the blocking agent.
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||1/200. PubMed: 18974869|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
FunctionProteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Tissue specificityDetected only in malignant melanoma cells.
Sequence similaritiesContains 15 CSPG (NG2) repeats.
Contains 2 laminin G-like domains.
modificationsO-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
Cellular localizationApical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
- Information by UniProt
- 4732461B14Rik antibody
- AN2 antibody
- AN2 proteoglycan antibody
ab76897 staining NG2 in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 5% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/250) for 16 hours at 4°C. An Alexa Fluor®488 -conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
ab50009 staining NG2 in an immunoreactive cell (rat hippocampal) by Immunohistochemistry (PFA fixed).
Brains were postfixed (2–6 hours) in 4% PFA and placed in 30% sucrose (2 days) and sectioned (20–30 µm) with a cryostat. ab50009 was used at a 1/200 dilution. A goat anti-mouse-Alexa 594, 1/500, was used as the secondary antibody.
All lanes : Anti-NG2 antibody [132.38] (ab50009) at 1 µg/ml
Lane 1 : Rat Brain Tissue Lysate
Lane 2 : Rat Spinal Cord Tissue Lysate
Lane 3 : Rat Hippocampus Tissue Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 251 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa (possible non-specific binding), 41 kDa (possible non-specific binding)
Exposure time: 20 minutes
This product has been referenced in:
- Hamdan H et al. The wmN1 enhancer region in intron 1 is required for expression of human PLP1. Glia N/A:N/A (2018). Read more (PubMed: 29683207) »
- Zhang R et al. RGMa mediates reactive astrogliosis and glial scar formation through TGFß1/Smad2/3 signaling after stroke. Cell Death Differ 25:1503-1516 (2018). Read more (PubMed: 29396549) »