Product nameAnti-NG2 antibody [132.38] - BSA and Azide free
See all NG2 primary antibodies
DescriptionMouse monoclonal [132.38] to NG2 - BSA and Azide free
Tested applicationsSuitable for: WB, IP, ELISA, IHC-FoFr, IHC-Frmore details
Species reactivityReacts with: Rat
Does not react with: Mouse
Tissue, cells or virus corresponding to NG2.
- WB: Rat brain, spinal cord and hippocampus tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab177025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Predicted molecular weight: 251 kDa.
Abcam recommends using BSA as the blocking agent.
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionProteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Tissue specificityDetected only in malignant melanoma cells.
Sequence similaritiesContains 15 CSPG (NG2) repeats.
Contains 2 laminin G-like domains.
modificationsO-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
Cellular localizationApical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
- Information by UniProt
- 4732461B14Rik antibody
- AN2 antibody
- AN2 proteoglycan antibody
All lanes : Anti-NG2 antibody [132.38] (ab50009) at 1 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat spinal cord tissue lysate
Lane 3 : Rat hippocampus tissue lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 251 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
ab177025 has not yet been referenced specifically in any publications.