Overview

  • Product name

    Anti-NG2 antibody [132.38] - BSA and Azide free
    See all NG2 primary antibodies
  • Description

    Mouse monoclonal [132.38] to NG2 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-FoFr, IHC-Frmore details
  • Species reactivity

    Reacts with: Rat
    Does not react with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to NG2.

  • Positive control

    • WB: Rat brain, spinal cord and hippocampus tissue lysate.
  • General notes

    ab177025 is a PBS-only buffer format of ab50009.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab177025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 251 kDa.

Abcam recommends using BSA as the blocking agent.

 

IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
  • Tissue specificity

    Detected only in malignant melanoma cells.
  • Sequence similarities

    Contains 15 CSPG (NG2) repeats.
    Contains 2 laminin G-like domains.
  • Post-translational
    modifications

    O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
    Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
  • Cellular localization

    Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4732461B14Rik antibody
    • AN2 antibody
    • AN2 proteoglycan antibody
    • Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody
    • Chondroitin sulfate proteoglycan 4 antibody
    • Chondroitin sulfate proteoglycan NG2 antibody
    • Cspg4 antibody
    • Cspg4 chondroitin sulfate proteoglycan 4 antibody
    • CSPG4_HUMAN antibody
    • HMW-MAA antibody
    • HSN tumor-specific antigen antibody
    • Kiaa4232 antibody
    • MCSP antibody
    • MCSPG antibody
    • MEL-CSPG antibody
    • Melanoma chondroitin sulfate proteoglycan antibody
    • Melanoma-associated chondroitin sulfate proteoglycan antibody
    • MELCSPG antibody
    • MSK16 antibody
    • NG2 antibody
    see all

Images

  • All lanes : Anti-NG2 antibody [132.38] (ab50009) at 1 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat spinal cord tissue lysate
    Lane 3 : Rat hippocampus tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 251 kDa
    Observed band size: 270 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes

References

ab177025 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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