Overview

  • Product name

    Anti-NG2 antibody [EPR9195]
    See all NG2 primary antibodies
  • Description

    Rabbit monoclonal [EPR9195] to NG2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human NG2 aa 2250 to the C-terminus (C terminal).

  • Positive control

    • WB: A375 cell lysate. IHC-P: Human blood vessels and human melanoma tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab139406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 251 kDa.

For unpurified format use at 1/1000 - 1/10000.

IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
    • Tissue specificity

      Detected only in malignant melanoma cells.
    • Sequence similarities

      Contains 15 CSPG (NG2) repeats.
      Contains 2 laminin G-like domains.
    • Post-translational
      modifications

      O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
      Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
    • Cellular localization

      Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
    • Information by UniProt
    • Database links

    • Alternative names

      • 4732461B14Rik antibody
      • AN2 antibody
      • AN2 proteoglycan antibody
      • Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody
      • Chondroitin sulfate proteoglycan 4 antibody
      • Chondroitin sulfate proteoglycan NG2 antibody
      • Cspg4 antibody
      • Cspg4 chondroitin sulfate proteoglycan 4 antibody
      • CSPG4_HUMAN antibody
      • HMW-MAA antibody
      • HSN tumor-specific antigen antibody
      • Kiaa4232 antibody
      • MCSP antibody
      • MCSPG antibody
      • MEL-CSPG antibody
      • Melanoma chondroitin sulfate proteoglycan antibody
      • Melanoma-associated chondroitin sulfate proteoglycan antibody
      • MELCSPG antibody
      • MSK16 antibody
      • NG2 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labelling NG2 with unpurified ab139406 at 1/250 dilution.

    • Anti-NG2 antibody [EPR9195] (ab139406) at 1/2000 dilution (purified) + A375 (Human malignant melanoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 251 kDa
      Observed band size: 270 kDa
      why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human melanoma tissue sections labeling NG2 with purified  ab139406 at 1:400 dilution (1.33 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using citrate Buffer, pH6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Immunohistochemical analysis of paraffin-embedded Human blood vessel tissue labelling NG2 with unpurified ab139406 at 1/250 dilution.

    • Anti-NG2 antibody [EPR9195] (ab139406) at 1/1000 dilution (unpurified) + A375 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 251 kDa

    References

    This product has been referenced in:

    • Schöttelndreier D  et al. Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B. Front Microbiol 9:1483 (2018). Read more (PubMed: 30022975) »
    • Xu L  et al. Different Angiogenic Potentials of Mesenchymal Stem Cells Derived from Umbilical Artery, Umbilical Vein, and Wharton's Jelly. Stem Cells Int 2017:3175748 (2017). Read more (PubMed: 28874910) »
    See all 3 Publications for this product

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