Recombinant Anti-NG2 antibody [EPR9195] (ab139406)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9195] to NG2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NG2 antibody [EPR9195]
See all NG2 primary antibodies -
Description
Rabbit monoclonal [EPR9195] to NG2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human NG2 aa 2250 to the C-terminus (C terminal).
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Positive control
- WB: A375 cell lysate. IHC-P: Human blood vessels and human melanoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9195 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000. Predicted molecular weight: 251 kDa.
For unpurified format use at 1/1000 - 1/10000. |
IHC-P | (2) |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000. Predicted molecular weight: 251 kDa. For unpurified format use at 1/1000 - 1/10000. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. -
Tissue specificity
Detected only in malignant melanoma cells. -
Sequence similarities
Contains 15 CSPG (NG2) repeats.
Contains 2 laminin G-like domains. -
Post-translational
modificationsO-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility. -
Cellular localization
Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes. - Information by UniProt
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Database links
- Entrez Gene: 1464 Human
- Omim: 601172 Human
- SwissProt: Q6UVK1 Human
- Unigene: 513044 Human
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Alternative names
- 4732461B14Rik antibody
- AN2 antibody
- AN2 proteoglycan antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labelling NG2 with unpurified ab139406 at 1/250 dilution.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-NG2 antibody [EPR9195] (ab139406) at 1/2000 dilution (purified) + A375 (Human malignant melanoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 251 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human melanoma tissue sections labeling NG2 with purified ab139406 at 1:400 dilution (1.33 μg/ml). Heat mediated antigen retrieval was performed using citrate Buffer, pH6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemical analysis of paraffin-embedded Human blood vessel tissue labelling NG2 with unpurified ab139406 at 1/250 dilution.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-NG2 antibody [EPR9195] (ab139406) at 1/1000 dilution (unpurified) + A375 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 251 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab139406 has been referenced in 7 publications.
- Egan CE et al. CSPG4 Is a Potential Therapeutic Target in Anaplastic Thyroid Cancer. Thyroid N/A:N/A (2021). PubMed: 34078123
- Quintela-Fandino M et al. Randomized Phase 0/I Trial of the Mitochondrial Inhibitor ME-344 or Placebo Added to Bevacizumab in Early HER2-Negative Breast Cancer. Clin Cancer Res 26:35-45 (2020). PubMed: 31597662
- Quintela-Fandino M et al. Immuno-priming durvalumab with bevacizumab in HER2-negative advanced breast cancer: a pilot clinical trial. Breast Cancer Res 22:124 (2020). PubMed: 33176887
- Li Z et al. Transplantation of human endometrial perivascular cells with elevated CYR61 expression induces angiogenesis and promotes repair of a full-thickness uterine injury in rat. Stem Cell Res Ther 10:179 (2019). PubMed: 31215503
- Schöttelndreier D et al. Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B. Front Microbiol 9:1483 (2018). PubMed: 30022975
- Xu L et al. Different Angiogenic Potentials of Mesenchymal Stem Cells Derived from Umbilical Artery, Umbilical Vein, and Wharton's Jelly. Stem Cells Int 2017:3175748 (2017). PubMed: 28874910
- Tao L et al. Frizzled proteins are colonic epithelial receptors for C. difficile toxin B. Nature 538:350-355 (2016). PubMed: 27680706