Recombinant
RabMAb

Recombinant Anti-NG2 antibody [EPR9195] - BSA and Azide free (ab240112)

Overview

  • Product name

    Anti-NG2 antibody [EPR9195] - BSA and Azide free
    See all NG2 primary antibodies
  • Description

    Rabbit monoclonal [EPR9195] to NG2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NG2 aa 2250 to the C-terminus (C terminal). The exact sequence is proprietary.

  • General notes

    ab240112 is the carrier-free version of ab139406 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240112 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB Use at an assay dependent concentration. Predicted molecular weight: 251 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
    • Tissue specificity

      Detected only in malignant melanoma cells.
    • Sequence similarities

      Contains 15 CSPG (NG2) repeats.
      Contains 2 laminin G-like domains.
    • Post-translational
      modifications

      O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
      Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
    • Cellular localization

      Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
    • Information by UniProt
    • Database links

    • Alternative names

      • 4732461B14Rik antibody
      • AN2 antibody
      • AN2 proteoglycan antibody
      • Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody
      • Chondroitin sulfate proteoglycan 4 antibody
      • Chondroitin sulfate proteoglycan NG2 antibody
      • Cspg4 antibody
      • Cspg4 chondroitin sulfate proteoglycan 4 antibody
      • CSPG4_HUMAN antibody
      • HMW-MAA antibody
      • HSN tumor-specific antigen antibody
      • Kiaa4232 antibody
      • MCSP antibody
      • MCSPG antibody
      • MEL-CSPG antibody
      • Melanoma chondroitin sulfate proteoglycan antibody
      • Melanoma-associated chondroitin sulfate proteoglycan antibody
      • MELCSPG antibody
      • MSK16 antibody
      • NG2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human melanoma tissue sections labeling NG2 with purified  ab139406 at 1:400 dilution (1.33 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using citrate Buffer, pH6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab139406).

    • Immunohistochemical analysis of paraffin-embedded Human blood vessel tissue labelling NG2 with unpurified ab139406 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab139406).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labelling NG2 with unpurified ab139406 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab139406).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab240112 has not yet been referenced specifically in any publications.

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