Recombinant Anti-NG2 antibody [EPR9195] - BSA and Azide free (ab240112)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR9195] - BSA and Azide free (ab240112)](https://www.abcam.com/ps/products/240/ab240112/Images/ab240112-326784-anti-ng2-antibody-epr9195-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR9195] - BSA and Azide free (ab240112)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9195] to NG2 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-NG2 antibody [EPR9195] - BSA and Azide free
See all NG2 primary antibodies -
Description
Rabbit monoclonal [EPR9195] to NG2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NG2 aa 2250 to the C-terminus (C terminal). The exact sequence is proprietary.
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General notes
ab240112 is the carrier-free version of ab139406 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab240112 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9195 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab240112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 251 kDa. |
Target
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Function
Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. -
Tissue specificity
Detected only in malignant melanoma cells. -
Sequence similarities
Contains 15 CSPG (NG2) repeats.
Contains 2 laminin G-like domains. -
Post-translational
modificationsO-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility. -
Cellular localization
Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes. - Information by UniProt
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Database links
- Entrez Gene: 1464 Human
- Omim: 601172 Human
- SwissProt: Q6UVK1 Human
- Unigene: 513044 Human
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Alternative names
- 4732461B14Rik antibody
- AN2 antibody
- AN2 proteoglycan antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR9195] - BSA and Azide free (ab240112)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human melanoma tissue sections labeling NG2 with purified ab139406 at 1:400 dilution (1.33 μg/ml). Heat mediated antigen retrieval was performed using citrate Buffer, pH6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab139406).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR9195] - BSA and Azide free (ab240112)](https://www.abcam.com/ps/products/240/ab240112/Images/ab240112-326783-anti-ng2-antibody-epr9195-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR9195] - BSA and Azide free (ab240112)Immunohistochemical analysis of paraffin-embedded Human blood vessel tissue labelling NG2 with unpurified ab139406 at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab139406).
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR9195] - BSA and Azide free (ab240112)](https://www.abcam.com/ps/products/240/ab240112/Images/ab240112-326782-anti-ng2-antibody-epr9195-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR9195] - BSA and Azide free (ab240112)Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labelling NG2 with unpurified ab139406 at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab139406).
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-NG2 antibody [EPR9195] - BSA and Azide free (ab240112)](https://www.abcam.com/ps/products/240/ab240112/Images/ab240112-4-benefits-of-recombinant-antibodies.png)
Anti-NG2 antibody [EPR9195] - BSA and Azide free (ab240112)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab240112 has not yet been referenced specifically in any publications.
