Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8668] to NgBR - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-NgBR antibody [EPR8668] - BSA and Azide free
See all NgBR primary antibodies
DescriptionRabbit monoclonal [EPR8668] to NgBR - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human NgBR. The exact sequence is proprietary.
- WB: HeLa, HUVEC and A549 cell lysates.
ab236072 is the carrier-free version of ab168351 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab236072 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody was developed as part of a collaboration between Epitomics, the National Institutes of Health and the lab of Qing (Robert) Miao from the Medical College of Wisconsin. This product was previously labelled as Nogo B receptor
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab236072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.|
FunctionActs as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator. Regulates the stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol.
Essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the UPP synthase family.
Cellular localizationEndoplasmic reticulum membrane. Colocalizes with Nogo-B during VEGF and wound healing angiogenesis.
- Information by UniProt
- C6orf68 antibody
- MGC:7199 antibody
- NgBR antibody
All lanes : Anti-NgBR antibody [EPR8668] (ab168351) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168351).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236072 has not yet been referenced specifically in any publications.