Key features and details
- Rabbit polyclonal to NGDN
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NGDN antibody
DescriptionRabbit polyclonal to NGDN
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- NGDN transfected HEK293T lysate, Human skin tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.|
FunctionInvolved in the translational repression of cytoplasmic polyadenylation element (CPE)-containing mRNAs.
Sequence similaritiesBelongs to the SAS10 family.
Cellular localizationNucleus. Cytoplasm. Cell projection > axon. Cell projection > dendrite. Cell projection > filopodium. Detected in axons, dendrites and filopodia. Colocalized with EIF4E in neurites.
- Information by UniProt
- C14orf120 antibody
- EIF4E-binding protein antibody
- Neuroguidin antibody
Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus, nucleoli & mitochondria.
All lanes : Anti-NGDN antibody (ab121124) at 1/250 dilution
Lane 1 : vector only transfected HEK293T lysate
Lane 2 : NGDN-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Developed using the ECL technique.
10-20% SDS PAGE
ab121124 at 1/350 dilution staining NGDN in Paraffin-emebedded Human skin tissue by Immunohistochemistry. Note: strong nuclear positivity in epidermal cells.
ab121124 has not yet been referenced specifically in any publications.