Product nameAnti-NGF antibody
See all NGF primary antibodies
DescriptionRabbit polyclonal to NGF
SpecificityLess than 1% cross-reactivity against recombinant human Brain Derived Neurotrophic Factor, Neurotrophin 3 and Neurotrophin 4/5 by ELISA. This NGF antibody detects a band of approximate molecular weight of 13KDa under denaturing conditions. This corresponds the monomer of beta NGF (as it exists as a dimer in the native state).
Tested applicationsSuitable for: Dot blot, ELISA, IHC-Fr, Neutralising, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human
Does not react with: Cow
Native mouse salivary gland NGF purified by modification of the method of Mobley et al (1976). Antigen purity was greater than 95% by PAGE.
General notesThis antibody has been shown to be useful for a variety of techniques and its specificity has been demonstrated by immunoblot.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Our Abpromise guarantee covers the use of ab6198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.|
|IHC-Fr||1/400 - 1/1000.|
|Neutralising||1/2 - 1/10.|
|WB||1/400 - 1/1000.|
|IHC-P||1/400 - 1/1000.|
FunctionNerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
Involvement in diseaseNeuropathy, hereditary sensory and autonomic, 5
Sequence similaritiesBelongs to the NGF-beta family.
- Information by UniProt
- Beta nerve growth factor antibody
- Beta NGF antibody
- Beta-nerve growth factor antibody
ab6198 at 1/1000 staining perfusion fixed rat brain tissue sections by IHC-Fr. The animals were pre-perfused with Tris buffer pH 10, followed by 4% paraformadehyde and 15% of a saturated solution of picric acid. The brains were post-fixed in the same fixative overnight, cryoprotected in 20% sucrose for 24 hours, frozen and cut with a cryostat. Free floating immunostaining was performed. An Alexa-Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary.
The image shows the staining obtained with this antibody using direct fluorescence in rat cortical neurons, with two different magnifications of acquisition (X10 and X20).
This product has been referenced in:
- Wang L et al. Tadalafil Promotes the Recovery of Peripheral Neuropathy in Type II Diabetic Mice. PLoS One 11:e0159665 (2016). IHC-P . Read more (PubMed: 27438594) »
- Messer HG et al. Inhibition of H3K27me3-specific histone demethylases JMJD3 and UTX blocks reactivation of herpes simplex virus 1 in trigeminal ganglion neurons. J Virol 89:3417-20 (2015). Read more (PubMed: 25552720) »