Overview

  • Product name
  • Description
    Rabbit polyclonal to NGF
  • Host species
    Rabbit
  • Specificity
    Less than 1% cross-reactivity against recombinant human Brain Derived Neurotrophic Factor, Neurotrophin 3 and Neurotrophin 4/5 by ELISA. This NGF antibody detects a band of approximate molecular weight of 13KDa under denaturing conditions. This corresponds the monomer of beta NGF (as it exists as a dimer in the native state).
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-Fr, Neutralising, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
    Does not react with: Cow
  • Immunogen

    Native mouse salivary gland NGF purified by modification of the method of Mobley et al (1976). Antigen purity was greater than 95% by PAGE.

  • General notes
    This antibody has been shown to be useful for a variety of techniques and its specificity has been demonstrated by immunoblot.

Properties

Applications

Our Abpromise guarantee covers the use of ab6198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA 1/3000.
IHC-Fr 1/400 - 1/1000.
Neutralising 1/2 - 1/10.
WB 1/400 - 1/1000.
IHC-P 1/400 - 1/1000.

Target

  • Function
    Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
  • Involvement in disease
    Neuropathy, hereditary sensory and autonomic, 5
  • Sequence similarities
    Belongs to the NGF-beta family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta nerve growth factor antibody
    • Beta NGF antibody
    • Beta-nerve growth factor antibody
    • Beta-NGF antibody
    • HSAN5 antibody
    • MGC161426 antibody
    • MGC161428 antibody
    • Nerve growth factor (beta polypeptide) antibody
    • Nerve growth factor antibody
    • Nerve growth factor beta antibody
    • Nerve growth factor beta polypeptide antibody
    • Nerve growth factor beta subunit antibody
    • NGF antibody
    • NGF_HUMAN antibody
    • NGFB antibody
    • NID67 antibody
    see all

Images

  • ab6198 at 1/1000 staining perfusion fixed rat brain tissue sections by IHC-Fr. The animals were pre-perfused with Tris buffer pH 10, followed by 4% paraformadehyde and 15% of a saturated solution of picric acid. The brains were post-fixed in the same fixative overnight, cryoprotected in 20% sucrose for 24 hours, frozen and cut with a cryostat. Free floating immunostaining was performed. An Alexa-Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary.

    The image shows the staining obtained with this antibody using direct fluorescence in rat cortical neurons, with two different magnifications of acquisition (X10 and X20).

    See Abreview

References

This product has been referenced in:
  • Wang L  et al. Tadalafil Promotes the Recovery of Peripheral Neuropathy in Type II Diabetic Mice. PLoS One 11:e0159665 (2016). IHC-P . Read more (PubMed: 27438594) »
  • Messer HG  et al. Inhibition of H3K27me3-specific histone demethylases JMJD3 and UTX blocks reactivation of herpes simplex virus 1 in trigeminal ganglion neurons. J Virol 89:3417-20 (2015). Read more (PubMed: 25552720) »
See all 8 Publications for this product

Customer reviews and Q&As

1-10 of 16 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12.5% gel)
Sample
Rat Tissue lysate - whole (bladder)
Specification
bladder
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 06 2013

Answer

Thank you for your reply, and I apologize for this inconvenience.

I certainly understand that ab9858 is not as useful to you as ab73680 would be. I can issue a credit to you.

The vial of ab8020 should be arriving shortly, please keep me updated about the results with this replacement, and let me know if there is anything else that you need!

Read More

Answer

Thank you again for your patience.

I apologize, but ab73680 is out of stock, and it may not be put back into stock for several months as recent lots have not passed quality control testing. Would you be interested in trying another vial of ab9669, or ab9858-

https://www.abcam.com/MCP1-antibody-ab9858.html

Please let me know and I will arrange for this to be sent to you promptly. Have a great day!

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Answer

Thank you for your reply.

Please keep me updated on any progress. I hope that we hear something from them soon.

Please let me know if you need anything else from me at this time, and I'll let you know if I hear from someone at BioConnect. You have a good weekend as well!

Read More

Answer

Thank you again for your patience.

At this point, I have not heard from Bio-Connect regarding these issues and I've looked through our system and don't see that they've corresponded with anyone else at Abcam. Have you received any update from them? Is there anyone at Bio-Connect who I can contact?

I look forward to hearing from you. I'll be out of the office Monday-Thursday of next week but I will be back in touch nextFriday if there is anything else that I can do to help resolve these issues. Thanks so much! Have a great weekend.

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Answer

Thank you for getting back to me and for getting in touch with BioConnect. I will let you know if I don't hear anything from them shortly, and we will figure out the vial of ab9669. I'll also send you a confirmation email after I work everything out with them.

Thank you for your understanding, and let me know if you have any questions in the meantime.

Read More

Answer

Thank you for your reply and for confirming that information.

I'm processing the refunds and replacements now. I think I've found your order for ab22048, ab9100, and ab8021, but I am having some trouble finding the order for ab9669. Were these ordered through our distributor in the Netherlands, Bio-Connect? If so, could you plrease forward me your shipping address and your Bio-Connect customer or account number, to make sure the refunds and replacements reach you.

For the MCP1 antibody, is it possible that you currently have a different antibody other than ab9669? Our records only show one order of ab9669 shipped to the Netherlands and that was placed in 2008. If you could find out the Abcam order number from your contact at Bio-Connect, it would be very helpful.

I am sorry for the delay in processing these, but I just want to make sure you are properly reimbursed. I look forward to hearing from you, and please let me know if you have any questions or further information. I hope you enjoy your weekend as well!

Read More

Answer

Thank you for getting back to me with your selections.

For CX3CR1, we do have a couple of other anti-human antibodies that have been tested in IHC on FFPE sections, ab8020 and ab51668-

https://www.abcam.com/CX3CR1-antibody-ab8020.html

https://www.abcam.com/CX3CR1-antibody-ab51668.html

Would you be interested in trying one of these as a replacement for ab8021? If not, I will go ahead and proceed with the refund. I will process your requests as soon as I hear back from you!

I look forward to hearing from you. Please let me know if you need anything else, and have a great day!

Read More

Answer

Thank you for getting back to me, and I am happy to help resolve this issue. I look forward to hearing from you, and please let me know if you would like any assistance selecting replacements, or if you have any other questions.

Have a great day!

Read More

Question

Dear sir, madam,

april 2011 our lab ordered the following abcam antibodies

anti-human Cx3Cr1 (ab8021)

anti-human BDNF (ab72439)

anti-human TLR4 (ab22048)

anti-human TLR2 (ab9100)

anti-human MCP-1(ab9669)

anti-human NGF (ab6198)

anti-human TGF-beta (ab66043)

Of these, BDNF, NGF, TGF seem to work fine. However, after using various human tissue imbedded paraffine sections (tonsil, skin psoriasis, lymphnode, and brain sepsis) we still get no (TLR2) and vague/unspecific (MCP1,CX3CR1, TLR4) immunoreactivity (IR).

The most concentrated dilution suggested for these antibodies was used, and antigen retrieval was performed according to protocol (for example TLR4: antigen retrieval with citrate buffer PH6).
The reason I haven't contacted you before, is because I wanted to test the markers on various human tissue which I first had to gather.

Please find images attached in a 'We Tranfer file' of TLR2, TLR4, MCP1 and Cx3Cr1 IHC of cortex sepsis, skin psoriasis, tonsil and lymphnode human paraffine embedded tissue (Images were too big to attach in this email, even when compressed).

I would like your expert opinion on these stainings.
In our opninion
TLR2 shows no immunoreactivity at all (note:TLR2 datasheet explicitly states lymphnode as tissue specificity).
TLR4 shows vessel-staining in lymphnode and cortex, and broader staining in tonsil and psoriasis. However doubtdful on the specificty.
MCP1 shows a diffuse staining pattern, which again may be unspecific?
CX3CR1 shows no immunoreactivity in sepsis cortex nor lymphnode, but some IR in tonsil in psoriasis.

Since these 4 antibodies do not perform as described on the datasheet, I wanted to ask for either a refund or a replacement of these products with Anti-TLR2 (ab24192), Anti-TLR4 (ab13556), Anti-MCP1 (ab73680) and a different batch of the same CxCr1 (ab8021).

Read More
Answer

Thank you for contacting us and letting us know about the trouble with these antibodies.

After reviewing the protocol and images, I agree that these antibodies might not be performing as expected. Our guarantee period generally extends for 6 months after purchase, but I understand that it takes time to thoroughly test so many antibodies, and so I would be happy to send the replacements that you mention or to issue refund. Would prefer a replacement or refund, and could you send me your original order number or PO number?

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

Read More

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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