Key features and details
- Rabbit polyclonal to NGF
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NGF antibody
See all NGF primary antibodies
DescriptionRabbit polyclonal to NGF
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Human
Highly pure (>98%) recombinant hNGFb (human Nerve Growth Factor beta).
- WB: Recombinant human NGF protein (ab9796).
FormLyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
No preservative, sterile filtered
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab9795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.
Detection limit for recombinant human NGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml.
Use 100 μl/well antibody solution. Detects at least 0.2 - 0.4 ng/well of recombinant human NGF.
FunctionNerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
Involvement in diseaseNeuropathy, hereditary sensory and autonomic, 5
Sequence similaritiesBelongs to the NGF-beta family.
- Information by UniProt
- Beta nerve growth factor antibody
- Beta NGF antibody
- Beta-nerve growth factor antibody
All lanes: Anti-NGF antibody (ab9795) at 0.2 µg/ml
Lane 1: Marker
Unreduced Recombinant human NGF protein (Active) (ab9796):
Lane 2: 250 ng
Lane 3: 125 ng
Lane 4: 62.5 ng
Lane 5: 31.25 ng
Lane 6: 15.625 ng
Lane 7: 7.8 ng
Lane 8: 3.9 ng
Lane 9: 1.95 ng
Lane 10: 0.975 ng
Lane 11: 0.4875 ng
Lane 12: 0.24 ng
To detect Human beta-NGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Rabbit polyclonal to NGF (Biotin) (ab271277) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human β-NGF.
ab9795 has been referenced in 1 publication.
- Ferrari MP et al. Safety and pharmacokinetics of escalating doses of human recombinant nerve growth factor eye drops in a double-masked, randomized clinical trial. BioDrugs 28:275-83 (2014). PubMed: 24327173