Product nameAnti-NGF antibody (Biotin)
See all NGF primary antibodies
DescriptionRabbit polyclonal to NGF (Biotin)
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
- WB: Recombinant human NGF.
FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Constituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271277 in the following tested applications.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 26 kDa.
When used in conjunction with compatible secondary reagents, the detection limit for recombinant human NGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
|ELISA||Use a concentration of 0.25 - 1 µg/ml.
Human NGF was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271277. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant NGF protein.
FunctionNerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
Involvement in diseaseNeuropathy, hereditary sensory and autonomic, 5
Sequence similaritiesBelongs to the NGF-beta family.
- Information by UniProt
- Beta nerve growth factor antibody
- Beta NGF antibody
- Beta-nerve growth factor antibody
Lane 1: Marker.
Lane 2: Recombinant hNGF at 250ng/lane.
Lane 3: Recombinant hNGF at 125ng/lane.
Lane 4: Recombinant hNGF at 62.5ng/lane.
Lane 5: Recombinant hNGF at 31.25ng/lane.
Lane 6: Recombinant hNGF at 15.625ng/lane.
Lane 7: Recombinant hNGF at 7.8ng/lane.
Lane 8: Recombinant hNGF at 3.9ng/lane.
Lane 9: Recombinant hNGF at 1.95ng/lane.
Lane 10: Recombinant hNGF at 0.975ng/lane.
Lane 11: Recombinant hNGF at 0.4875ng/lane.
Lane 12: Recombinant hNGF at 0.24ng/lane.
Human NGF was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271277. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least XXXX of recombinant NGF protein.
ab271277 has not yet been referenced specifically in any publications.