• Product name

    Anti-NGF antibody [EP1318Y]
    See all NGF primary antibodies
  • Description

    Rabbit monoclonal [EP1318Y] to NGF
  • Host species

  • Specificity

    This antibody recognizes pro-NGF.
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NGF aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Human pro-NGF recombinant protein
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab68151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 27 kDa.

For unpurified use at 1/1000 - 1/5000.

Flow Cyt 1/200 - 1/280.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
    • Involvement in disease

      Neuropathy, hereditary sensory and autonomic, 5
    • Sequence similarities

      Belongs to the NGF-beta family.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • Beta nerve growth factor antibody
      • Beta NGF antibody
      • Beta-nerve growth factor antibody
      • Beta-NGF antibody
      • HSAN5 antibody
      • MGC161426 antibody
      • MGC161428 antibody
      • Nerve growth factor (beta polypeptide) antibody
      • Nerve growth factor antibody
      • Nerve growth factor beta antibody
      • Nerve growth factor beta polypeptide antibody
      • Nerve growth factor beta subunit antibody
      • NGF antibody
      • NGF_HUMAN antibody
      • NGFB antibody
      • NID67 antibody
      see all


    • Overlay histogram showing SH-SY5Y cells stained with ab68151 (red line) at 1/280 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line)

    • Anti-NGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Rat brain lysate at 10 µg

      Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size: 27 kDa

    • Anti-NGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Human fetal brain lysate at 10 µg

      Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

      Predicted band size: 27 kDa

    • All lanes : Anti-NGF antibody [EP1318Y] (ab68151) at 1/5000 dilution (unpurified)

      Lane 1 : 10ng human pro-NGF recombinant protein
      Lane 2 : 5ng human pro-NGF recombinant protein
      Lane 3 : 1ng human pro-NGF recombinant protein
      Lane 4 : 0.5ng human pro-NGF recombinant protein

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 27 kDa
      Observed band size: 27 kDa
      Additional bands at: 13 kDa, 20 kDa. We are unsure as to the identity of these extra bands.


    This product has been referenced in:

    • Protto V  et al. Electroacupuncture in rats normalizes the diabetes-induced alterations in the septo-hippocampal cholinergic system. Hippocampus N/A:N/A (2019). Read more (PubMed: 30870587) »
    • Cai H  et al. Neuroprotective effects of bajijiasu against cognitive impairment induced by amyloid-ß in APP/PS1 mice. Oncotarget 8:92621-92634 (2017). Read more (PubMed: 29190943) »
    See all 2 Publications for this product

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