Overview

  • Product name

    Anti-NGF antibody [EP1320Y]
    See all NGF primary antibodies
  • Description

    Rabbit monoclonal [EP1320Y] to NGF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NGF aa 200-300. The exact sequence is proprietary.
    (Peptide available as ab154297)

  • Positive control

    • Human brain, fetal thyroid tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/300.
WB 1/1000. Predicted molecular weight: 27 kDa.

For unpurified, use 1/500.

IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocol.

Target

  • Function

    Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
  • Involvement in disease

    Neuropathy, hereditary sensory and autonomic, 5
  • Sequence similarities

    Belongs to the NGF-beta family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta nerve growth factor antibody
    • Beta NGF antibody
    • Beta-nerve growth factor antibody
    • Beta-NGF antibody
    • HSAN5 antibody
    • MGC161426 antibody
    • MGC161428 antibody
    • Nerve growth factor (beta polypeptide) antibody
    • Nerve growth factor antibody
    • Nerve growth factor beta antibody
    • Nerve growth factor beta polypeptide antibody
    • Nerve growth factor beta subunit antibody
    • NGF antibody
    • NGF_HUMAN antibody
    • NGFB antibody
    • NID67 antibody
    see all

Images

  • All lanes : Anti-NGF antibody [EP1320Y] (ab52918) at 1/1000 dilution (purified)

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal thymus lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Anti-NGF antibody [EP1320Y] (ab52918) at 1/1000 dilution (purified) + Mouse thyroid lysate at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 32 kDa why is the actual band size different from the predicted?



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Anti-NGF antibody [EP1320Y] (ab52918) at 1/2000 dilution (purified) + Rat thyroid lysate at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 32 kDa why is the actual band size different from the predicted?



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab52918 at a working dilution of 1 in 250. The secondary antibody used is ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunofluorescence staining of U87-MG cells with purified ab52918 at a working dilution of 1 in 300, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab52918 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

  • Anti-NGF antibody [EP1320Y] (ab52918) at 1/500 dilution (unpurified) + fetal thyroid tissue at 10 µg

    Secondary
    Goat anti rabbit IgG HRP antibody at 1/2000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa

  • Immunohistochemical staining of paraffin embedded human brain using unpurified b52918 at 1/50-1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemical staining of NGF mouse tissue sections (formalin/ PFA-fixed paraffin-embedded tissue sections) with unpurified ab52918. The sections were formaldehyde fixed, subjected to heat mediated antigen retrieval and blocked for 10 minutes at 25°C. The primary antibody was diluted 1/50 and incubated with the sample for 1 hour at 25°C. An HRP polymer anti-rabbit IgG system was used undiluted, as the secondary antibody.

     

    See Abreview

References

This product has been referenced in:

  • Nagura N  et al. IL-1ß mediates NGF and COX-2 expression through transforming growth factor-activating kinase 1 in subacromial bursa cells derived from rotator cuff tear patients. J Orthop Sci N/A:N/A (2019). Read more (PubMed: 30799163) »
  • Bortolini MAT  et al. Molecular and histomorphological evaluation of female rats' urethral tissues after an innovative trauma model of prolonged vaginal distention: immediate, short-term and long-term effects. Int Urogynecol J 30:465-476 (2019). Read more (PubMed: 29564513) »
See all 21 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Nerve)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
Permeabilization
No
Specification
Nerve
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 12 2018

Question
Answer

Product ab154297 can be used as the blocking peptide with ab52918.

Read More

Answer

Thank you for contacting us.

I am happy to confirm the blocking peptide for Ab52918 has now been added to our catalogue under reference ab154297.

I’d like to bring your attention to an offer we are running throughout November. If you order any primary antibody you can receive a RabMab free, whilst stocks last. Simply quote “RABMAB-XBSMG” in your next primary antibody order. More information on this offer can be found from the following link:

https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Please let me know if you require further assistance.

Read More

Answer

Thank you for your enquiry.

I can confirm that many of our antibodies are supplied in purified format, and we can provide the concentration forthese.

However, unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate. The concentration is therefore not determined.

As far as I am aware, this situation would be the same for any company selling antibodies.

I am able to provide an estimate. Generally, for tissue culture supernatant antibodies, concentration of antibody is known to vary between 1 - 3 mg/ml.

I do understand that an estimate does make this tricky when selecting a concentration to use the isotype control, however I hope this will help. Regrettably we do not currently have a rabbit monoclonal IgG isotype control supplied in TC supernatant format.

For future reference:
For whole antiserum, concentration of antibody is known to very between 1 - 10 mg/ml.
For ascites, concentration of antibody is known to very between 5 - 10 mg/ml.

I am sorry we are not able to provide an exact concentration for ab52918 on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse ear)
Specification
Mouse ear
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako S2375 High pH HIER
Permeabilization
No
Blocking step
(agent) for 10 minute(s) · Concentration: 100% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 14 2010

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