Product nameAnti-NHE-7 antibody
See all NHE-7 primary antibodies
DescriptionRabbit polyclonal to NHE-7
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Goat, Guinea pig, Dog, Zebrafish
- Jurkat cell lysate.
Previously labelled as SLC9A7.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab75184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
FunctionMediates electroneutral exchange of protons for Na(+) and K(+) across endomembranes. May contribute to Golgi volume and cation homeostasis.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
Cellular localizationGolgi apparatus, trans-Golgi network membrane. Recycling endosome membrane.
- Information by UniProt
- Na(+)/H(+) exchanger 7 antibody
- NHE-7 antibody
- NHE7 antibody
Anti-NHE-7 antibody (ab75184) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
ab75184 has not yet been referenced specifically in any publications.