Overview

  • Product name
  • Description
    Goat polyclonal to NHERF-2
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human NHERF-2 (internal sequence).
    Sequence:

    (C)DGSAWKQDPFQ


    Database link: BAA33216.1

  • Positive control
    • Human kidney lysate. Human kidney tissue sections.
  • General notes

    Previously labelled as SLC9A3R2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab40825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 37 kDa).
IHC-P Use a concentration of 1 - 2 µg/ml.

Target

  • Function
    Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Contains 2 PDZ (DHR) domains.
  • Cellular localization
    Endomembrane system. Nucleus. Apical cell membrane. Localizes with EZR and PODXL at the apical cell membrane of glomerular epithelium cells and the sides of the food processes (By similarity). Nuclear, in a punctate pattern.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3KARP antibody
    • HGNC:11076 antibody
    • Isoform 3 regulator 2 antibody
    • Isoform 3 regulatory factor 2 antibody
    • MGC104639 antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE RF2 antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE-RF2 antibody
    • NHE3 kinase A regulatory protein antibody
    • NHE3 kinase A regulatory protein E3KARP antibody
    • NHE3 regulatory factor 2 antibody
    • NHE3RF2 antibody
    • NHERF 2 antibody
    • NHERF-2 antibody
    • NHERF2 antibody
    • NHRF2_HUMAN antibody
    • OCTS2 antibody
    • SIP 1 antibody
    • SIP-1 antibody
    • SIP1 antibody
    • Slc9a3r2 antibody
    • Sodium hydrogen exchanger regulatory factor 2 antibody
    • Sodium-hydrogen exchanger regulatory factor 2 antibody
    • Sodium/hydrogen exchanger antibody
    • Sodium/hydrogen exchanger regulatory factor 2 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger) antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger) isoform 3 regulator 2 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger) isoform 3 regulatory factor 2 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 2 antibody
    • Solute carrier family 9 isoform 3 regulator 2 antibody
    • Solute carrier family 9 isoform A3 regulatory factor 2 antibody
    • SRY interacting protein 1 antibody
    • SRY-interacting protein 1 antibody
    • TKA 1 antibody
    • TKA-1 antibody
    • TKA1 antibody
    • Tyrosine kinase activator protein 1 antibody
    see all

Images

  • Anti-NHERF-2 antibody (ab40825) at 0.3 µg/ml + Human Kidney lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 37 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

  • ab40825 at 3ug/ml staining NHERF-2 in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.

References

This product has been referenced in:
  • Filippov AK  et al. The scaffold protein NHERF2 determines the coupling of P2Y1 nucleotide and mGluR5 glutamate receptor to different ion channels in neurons. J Neurosci 30:11068-72 (2010). WB, ICC/IF . Read more (PubMed: 20720114) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab40825 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support but please bear in mind that this product has not been tested with rat samples and might not be suitable for use. However, I have looked through the protocols you used and have a few questions and suggestions that might help you resolve the problem. In most cases, the cause of multiple bands is because the amount of protein is too much. We normally recommend using 20-40 micrograms per well. Please try this amount if you have not already done so. Loading more than this may overload the gel, which will result in many non-specific bands. The bands located above the expected molecular weight band could be multimers. Did you boil your protein in SDS-PAGE for 10 minutes? This will ensure the protein is in the correct conformation to run at the correct molecular weight and be detected by the antibody. Meanwhile, bands located below the expected molecular weight may indicate that your target protein has been digested. Please make sure that you incorporated sufficient protease inhibitors and have kept your samples on ice the whole time. You can also run a no-primary control (without the primary antibody). Running a no-primary control will be able to eliminate the possibility that your secondary antibody is binding non-specifically and causing the bands at 50kDa and 110kDa. If the above suggestions did not help to improve your results, then I am afraid that this product is indeed not suitable for use with rat species. In any case, can I encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to other researchers. We reward each Abreview with Abpoints which can be used for discounts off future purchases and gifts. If you decide to submit an Abreview, please include a note that you have already spoken to me, so that I will be able to quickly publish your Abreview, as I have already tried troubleshooting with you. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews Should you require any further information or assistance, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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