Key features and details
- Mouse monoclonal [32B6] to NHERF-2/SIP-1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-NHERF-2/SIP-1 antibody [32B6]
See all NHERF-2/SIP-1 primary antibodies
DescriptionMouse monoclonal [32B6] to NHERF-2/SIP-1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Full length protein corresponding to Human NHERF-2/SIP-1.
MAAPEPLRPRLCRLVRGEQGYGFHLHGEKGRRGQFIRRVEPGSPAEAAAL RAGDRLVEVNGVNVEGETHHQVVQRIKAVEGQTRLLVVDQETDEELRRRQ LTCTEEMAQRGLPPAHDPWEPKPDWAHTGSHSSEAGKKDVSGPLRELRPR LCHLRKGPQGYGFNLHSDKSRPGQYIRSVDPGSPAARSGLRAQDRLIEVN GQNVEGLRHAEVVASIKAREDEARLLVVDPETDEHFKRLRVTPTEEHVEG PLPSPVTNGTSPAQLNGGSACSSRSDLPGSDKDTEDGSAWKQDPFQESGL HLSPTAAEAKEKARAMRVNKRAPQMDWNRKREIFSNF
- WB: HEK293T, MCF7, T-47D and HaCat cell lysates.
Previously labelled as SLC9A3R2.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab151443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100. Predicted molecular weight: 37 kDa.|
FunctionScaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus.
Tissue specificityWidely expressed.
Sequence similaritiesContains 2 PDZ (DHR) domains.
Cellular localizationEndomembrane system. Nucleus. Apical cell membrane. Localizes with EZR and PODXL at the apical cell membrane of glomerular epithelium cells and the sides of the food processes (By similarity). Nuclear, in a punctate pattern.
- Information by UniProt
- E3KARP antibody
- HGNC:11076 antibody
- Isoform 3 regulator 2 antibody
All lanes : Anti-NHERF-2/SIP-1 antibody [32B6] (ab151443) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : SLC9A3R2 knockout HEK293T cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : T-47D cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Lanes 1-4: Merged signal (red and green). Green - ab151443 observed at 45 kDa. Red - loading control ab52901 observed at kDa.
ab151443 Anti-NHERF-2/SIP-1 antibody [32B6] was shown to specifically react with NHERF-2/SIP-1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266778 (knockout cell lysate ab258685) was used. Wild-type and NHERF-2/SIP-1 knockout samples were subjected to SDS-PAGE. ab151443 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Anti-NHERF-2/SIP-1 antibody [32B6] (ab151443) at 1/100 dilution + HaCat Human keratinocyte cells
Predicted band size: 37 kDa
ab151443 has not yet been referenced specifically in any publications.