Key features and details
- Rabbit polyclonal to NHLRC1/Malin
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-NHLRC1/Malin antibody
See all NHLRC1/Malin primary antibodies
DescriptionRabbit polyclonal to NHLRC1/Malin
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment corresponding to Human NHLRC1/Malin aa 1-280.
MAAEASESGPALHELMREAEISLLECKVCFEKFGHRQQRRPRNLSCGHVV CLACVAALAHPRTLALECPFCRRACRGCDTSDCLPVLHLIELLGSALRQS PAAHRAAPSAPGALTCHHTFGGWGTLVNPTGLALCPKTGRVVVVHDGRRR VKIFDSGGGCAHQFGEKGDAAQDIRYPVDVTITNDCHVVVTDAGDRSIKV FDFFGQIKLVIGGQFSLPWGVETTPQNGIVVTDAEAGSLHLLDVDFAEGV LRRTERLQAHLCNPRGVAVSWLTGAIAVLE
Database link: Q6VVB1
- WB: LO2 whole cell lysate; Rat liver lysate. IHC-P: Human heart and liver tissues.
This product was previously labelled as NHLRC1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
FunctionE3 ubiquitin-protein ligase. Together with the phosphatase EPM2A/laforin, appears to be involved in the clearance of toxic polyglucosan and protein aggregates via multiple pathways. In complex with EPM2A/laforin and HSP70, suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Ubiquitinates the glycogen-targeting protein phosphatase subunits PPP1R3C/PTG and PPP1R3D in a laforin-dependent manner and targets them for proteasome-dependent degradation, thus decreasing glycogen accumulation. Polyubiquitinates EPM2A/laforin and ubiquitinates AGL and targets them for proteasome-dependent degradation. Also promotes proteasome-independent protein degradation through the macroautophagy pathway.
Tissue specificityExpressed in brain, cerebellum, spinal cord, medulla, heart, liver, skeletal muscle and pancreas.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseEpilepsy, progressive myoclonic 2
Sequence similaritiesContains 6 NHL repeats.
Contains 1 RING-type zinc finger.
DomainThe RING domain is essential for ubiquitin E3 ligase activity.
Cellular localizationEndoplasmic reticulum. Nucleus. Localizes at the endoplasmic reticulum and, to a lesser extent, in the nucleus.
- Information by UniProt
- bA204B7.2 antibody
- E3 ubiquitin-protein ligase NHLRC1 antibody
- EPM2A antibody
Paraffin-embedded human heart tissue stained for NHLRC1/Malin using ab225919 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for NHLRC1/Malin using ab225919 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-NHLRC1/Malin antibody (ab225919) at 1/1000 dilution
Lane 1 : LO2 whole cell lysate
Lane 2 : Rat liver lysate
All lanes : Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225919 has not yet been referenced specifically in any publications.